Hydrophobic interaction-induced circular dichroism

Hydrophobic Interaction-Induced Circular Dichroism (HIICD)... [Pg.37]

A study with the hydrophobic signal peptide of coli lambda phage in phospholipid monolayers, showed a preference for pC helical conformation when the peptide was inserted into the lipid phase (18), However, interaction with the lipid surface without insertion induced the peptide to adopt the -structure ), These observations, obtained with circular dichroism and Fourier transform-infrared (FT-IR) data, provide the first direct evidence for interconversions between various conformational... [Pg.630]

Spectroscopic studies (fluorescence and circular dichroism) indicate that the variation in lipases stability is related to their hydrophobicity and therefore to the different degrees of interactions between the enzymes and the micellar interface [88]. Two important factors contribute to inducing conformational changes on the enzyme molecules in reverse micelles the unusual properties of the encapsulated water and the electrostatic interactions between ionic head groups of the surfactant and the polypeptide chains of the enzyme. Spectroscopic data on the structure of enzymes entrapped in reverse micelles are controversial, as regards the changes in the protein structure upon incorporation into reverse micelles (Table 13.3). [Pg.360]