Hydrophobic interaction chromatography operation

Minimized interaction between eluite molecules and column packing is operative in hydrophobic interaction chromatography where biological solutes are so mildly bound to a stationary phase that their delicate native tertiary structure is not spoiled. This separation of extreme mildness is also a separation of chemically differing species, i.e., separation by composition. 

This is a simple upgrade of the isocratic system with the facility for gradient elution techniques and greater functionality (Fig. 1.1(b)). The basic system provides for manual operating gradient techniques such as reversed phase, ion exchange and hydrophobic interaction chromatography. Any of the detectors listed above under the isocratic system can be used. 

Investigate conditions, which may be solvent compatible with previous and subsequent unit operations. Identify the appropriateness of normal-phase, revcrsed-pha.se. lon-exchanec or hydrophobic interaction chromatography as the desired mechanism. 

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