Hydrophobic interaction chromatograph Subject

After initial cell fermentation and product extraction from the producer cells, the crude preparation is subject to multiple chromatographic steps, including ion-exchange, hydrophobic interaction chromatography and gel-filtration chromatography. The purified product is presented in lyophilized form in vials (1 mg active/vial) and excipients include a phosphate buffer, sodium chloride and serum albumin. 

A number of fundamental questions related to chromatographic equilibria and dynamics have been explicated. The fundamental issue of the nature of the macromolecular dimensions that determine Ksfc s the subject of Chapter 1. The resolving power of aqueous SEC columns is considered from the perspective of column pore size distributions in Chapter 6. These and other aspects of efficiency are explored in detail in Chapter 7. Interactions between macromolecules and stationary phases are covered in both Chapter 2 (hydrophobic effects) and Chapter 3 (electrostatic effects). 

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