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Hydrophobic affinity chromatography

UDP-o-glucuronic acid (18) Hydrophobic affinity chromatography of collagen D-glucosyltransferases 122, 128... [Pg.533]

Hydrophobic affinity chromatography of chloroamphenicol acetyltransferases Purification of phenoloxidase by affinity... [Pg.437]

Hydrophobic affinity chromatography in 119 the fractionation of enzymes from halophilic bacteria... [Pg.440]

Affinity chromatography involves precisely the same kind of electrostatic, hydrophobic, dipolar, and hydrogen-bonding interactions described above, but the specificity of binding is extraordinarily high. Demands on the homogeneity of the stationary phase and on the rigidity of the support are often... [Pg.11]

Poly(HA) depolymerases - as far as it has been tested - do not bind to anion exchangers such as DEAE (at neutral pH) but have a pronounced affinity to hydrophobic materials. Therefore, many purification protocols include hydrophobic interaction chromatography. [Pg.298]

Gretch, D.R., Suter, M., and Stinski, M.F. (1987) The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpesvirus hydrophobic proteins or glycoproteins. Anal. Biochem. 163, 270-277. [Pg.1069]

Other methods that are related to affinity chromatography include hydrophobic interaction chromatography and thiophilic adsorption. The former is based on the interactions of proteins, peptides, and nucleic acids with short nonpolar chains on a support. This was first described in 1972 [113,114] following work that examined the role of spacer arms on the nonspecific adsorption of affinity columns [114]. Thiophilic adsorption, also known as covalent or chemisorption chromatography, makes use of immobilized thiol groups for solute retention [115]. Applications of this method include the analysis of sulfhydryl-containing peptides or proteins and mercurated polynucleotides [116]. [Pg.378]

The classification of chromatography as gel permeation chromatography (GPC size-exclusion chromatography, SEC gel filtration, GF), ion exchange chromatography (lEC), hydrophobic interaction chromatography (HIC), and affinity chromatography (AC) is... [Pg.91]

Ion exchange chromatography, hydrophobic interaction chromatography (HlC), and affinity chromatography (AC) show some similarities concerning practical realization therefore, most of the hints given for lEC are applicable for HlC and AC. Examples for AC are given in Protocols 3.6.2.4 (biospecific desorption) and 3.6.2.5 (elution by partial denaturation). [Pg.102]

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See also in sourсe #XX -- [ Pg.120 ]



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Affinity chromatography

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