Hydrophilic/hydrophobic copolymers plasma protein

The Kinetics of Adsorption of Plasma Proteins to a Hydrophilic-Hydrophobic Copolymers... 

Adsorption of Proteins from Plasma to a Series of Hydrophilic-hydrophobic Copolymers. II. Compositional Analysis with the Prelabeled Protein Technique... 

Horbett, T. A. The kinetics of adsorption of plasma proteins to a series of hydrophilic-hydrophobic copolymers, in Adhesion and Adsorption of Polymers, Part B (ed.) L. H. Lee, p. 677, New York, Plenum Publishing Corp. 1980... 

Lens hazing and protein deposition are common problems for wearers of soft contact lenses. Previous experiments with hydrophobic-hydrophilic copolymers exposed to plasma showed protein adsorption to be minimal at intermediate copolymer compositions. Adsorption of proteins from artificial tear solutions to a series of polymers and copolymers ranging in composition from 100% poly (methyl methacrylate) (PMMA) to 100% poly(2-hydroxyethyl methacrylate) (PH EM A) was measured. The total protein adsorption due to the three major proteins in tear fluid (lysozyme, albumin, and immunoglobulins) was at a minimum value at copolymer compositions containing 50% or less PH EM A. The elution of the adsorbed proteins from these polymers and copolymers with various solutions also was investigated to assess the binding mechanism. 

Little albumin and y-globulins were adsorbed on the MPC copolymer surface treated with DPPC, which is in sharp contrast to the fact that on a poly(BMA) surface, pretreatment with DPPC was not effective for suppression of protein adsorption (74). The difference in protein adsorption on these polymer surfaces reflects the difference in the orientation of the DPPC which covered the polymer surface. In Fig. 3, the adsorbed amounts of phospholipids, phosphatidylcholine (PC) on the polymers from human plasma is indicated (77). The amount of phospholipids adsorbed on hydrophobic poly(BMA) was almost the same as that on hydrophilic poly(HEMA). On poly(MPC-c< -BMA), a larger amount of phospholipids was adsorbed compared with poly(BMA), and the amount adsorbed increased with an increase in the MPC mole fraction of the copolymer. 

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