Protein from artificial tear solutions

Adsorption of Proteins from Artificial Tear Solutions to Poly(methyl methacrylate—2 -hydroxyethyl methacrylate) Copolymers... 

Lens hazing and protein deposition are common problems for wearers of soft contact lenses. Previous experiments with hydrophobic-hydrophilic copolymers exposed to plasma showed protein adsorption to be minimal at intermediate copolymer compositions. Adsorption of proteins from artificial tear solutions to a series of polymers and copolymers ranging in composition from 100% poly (methyl methacrylate) (PMMA) to 100% poly(2-hydroxyethyl methacrylate) (PH EM A) was measured. The total protein adsorption due to the three major proteins in tear fluid (lysozyme, albumin, and immunoglobulins) was at a minimum value at copolymer compositions containing 50% or less PH EM A. The elution of the adsorbed proteins from these polymers and copolymers with various solutions also was investigated to assess the binding mechanism. 

Adsorption of Proteins from Artificial Tear Solutions 457... 

Figure 2 summarizes the data for the three protein adsorption experiments performed. Each curve represents the amount of protein bound to the surface after the overnight soak rinse. The top curve indicates the total amount of protein adsorbed, and is simply a summation of the data for the three proteins studied. The curve was compiled using the data from three separate adsorption experiments, each of which examined one labeled protein at a time in the artificial tear solution. 

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