Hydrolases, use

Shimazu, M., Mulchandani, A. and Chen, W. (2001) Cell surface display of organophosphorus hydrolase using ice nucleation protein. Biotechnology Progress, 17, 76-80. 

R. S. Munford Jr., L. Eidels, and E. J. Hansen in A. Nowotny, J. J. Spitzer, and E. J. Ziegler (Eds.), Endotoxin Research Series, Vol. 1, Cellular and Molecular Aspects of Endotoxin Reactions Purification of Human Acyloxyacyl Hydrolase Using Affinity Chromatography, p. 305. Medica, Amsterdam/New York/Oxford, 1990. 

Turner, M. A., et al. (1998). Structure determination of selenomethionyl S-adenosyUiomocysteine hydrolase using data at a single wavelength. Nat. Struct. Biol. 5, 369-376. 

Efremenko, E., Votchitseva, Yu., Plieva, F., Galaev, T, and Mattiasson, B. (2006) Purifieation of Hisg-organophosphate hydrolase using monolithic supermacroporous polyacrylamide cryogels developed for immobilized metal affinity chromatography, Appl. Microbiol. Biot. 70(5) 558-563. 

Two broad strategies will concern us here. First, we will need to understand the two types of general chemical mechanism that hydrolases use and the heavy atom rearrangements that each entails. The two chemical mechanisms lead to differences... 

Varanasi, S., Rao, K., Relue, P. A Novel technique that enables efficient fermentation of xylose and hexose sugars from biomass hydrolasates using native non-GMO yeasts, US provisional patent application filed 01/4/08. 

Evans, G. O. 1990. Species relationships for plasma acylcholine hydrolase using three different substrates. In Fourth Congress of International Society for Animal Clinical Biochemistry, p. 270. University of California, Davis. 

HYDROLASES USING DIVALENT-DIVALENT DIMETAL CENTERS... 

Lipolytic enzymes are usually considered hydrolases using water as a cosubstrate. It should be noted, however, that most hydrolases act also as transferases when the cosubstrate is ROH, and several examples of this will be given in the following text. 

Artificial Hydrolase Using Modified Dimethyl- -cyclodextrin... 

For these reasons, if j -DMCD were used as an enzyme model, a new unique enzyme model might be obtained. In this paper, we wish to provide the first successful method for modification of j -DMCD and to investigate the enzymatic activities of a new artificial hydrolase using modified jS-DMCD. 

Dceda, H., R. Kojin, C. Yoon, T. Dceda, and F. Toda Artificial hydrolase using modified dimethyl-B-cyclodextrin 117... 

The catalysis itself can be infiuenced by the nature of the solvent. For instance, since the hydrolases used to catalyze condensation involve a charged and polar transition state, the tetrahedral intermediate depicted in Figure 7, it can be expected that a change in the polarity of the environment will affect the transition state and thus the kinetics. Indeed, Wangikar and co-workers observed a stabilization of the transition state for the transesterification of amino acid esters catalyzed by subtilisin BPN when going from hexane to the more polar acetone (47). 

The hydrolytic activity of PET hydrolases and the resulting increase in surface hydrophilicity of PET by the addition of non-ionic surfactants such as Triton X-100 have been evaluated. The observed effects appeared to depend on the type of PET hydrolase used. Treatment of PET fabrics with the lipase from T. insolens in combination with Triton X-100 resulted in a strong increase in the hydrophilicity of the PET material [62]. Likewise, hydrolysis of the PET trimer model compound with the lipase from T. lanuginosus was strongly increased in the presence of Triton X-100, which can be explained by the interfacial activation of lipases involved in the hydrolysis of PET substrates [27]. However, Triton X-100 did not increase the moisture regain of PET fabrics treated with a lipase from C. antarctica [60]. The hydrolytic activity of the PET hydrolases from T. fusca and F. solani were also not improved in the presence of Triton X-100 as can be expected from the lack of a lid structure and interfacial activation in cutinases [19, 27]. A considerable increase in the hydrolysis of PET fabrics and film by the... 

The aim of this study was the development of conversion system of caffeoylquinic acids to valuable compounds. When an IL, l-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([bmim][NTf2]) was used as a reaction solvent, we found that immobilized chlorogenate hydrolase (Kikkoman) catalyzed the conversion of 5-caffeoylquinic acid to methyl caffeate with methanol (Fig. 1). The immobilized enzyme was prepared with chlorogenate hydrolase using quaternary ammonium sepabeads (Mitsubishi Chemical Co., Tokyo, Japan) (Kurata et al., 2011). To synthesize valuable compounds from caffeoylquinic adds, we attempted to develop a method for the conversion of caffeoylquinic acids to CAPE analogues via methyl caffeate. In section 2, we describe the properties of immobilised chlorogenate hydrolase in ILs. Using various caffeoyl quinic acid prepared from immature coffee beans, we developed a system to produce methyl caffeate. 

Chen, X.-J., Archelas, A. and Furstoss, R. (1993) Microbiological transformations. 27. The first examples for preparative-scale enantioselective or diasteieoselective epoxide hydrolases using microorganisms. An unequivocal access to all four bisabolol stereoisomers. /. Org. Chem., 58, 5528-5532. 

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