Hydrogen-deuterium exchange polypeptides

Two auxiliary techniques, electron microscopy and hydrogen-deuterium exchange, as well as a relatively new method, far ultraviolet spectroscopy, also provide useful data on chain conformation in synthetic polypeptides. 

The capacity of rotatory dispersion to assess partial helical content in synthetic polypeptides has been demonstrated both by its self-consistency and general agreement with hydrogen-deuterium exchange. If this same pattern of analysis discerns the presence of helical segments in globular... 

The reader will find many references in this book to spectroscopy done on biochemical substances in aqueous solution. Chapters 6, 10, 11, 12, and 15 on carbohydrates, proteins and polypeptides, hydrogen-deuterium exchange, nucleic acids, and enzymology contain representative discussions of such studies. 

As seen in Chapter 11 (Hydrogen-Deuterium Exchange), infrared spectroscopy can be used conveniently for measuring the deuteration rate of biological polymers, e.g., polypeptides and proteins. Hydrogen isotopic exchange of DNA has been studied... 

Conformations of polypeptides may be elucidated by N NMR and details of useful techniques, including comparison with model compounds, hydrogen-deuterium exchange, and solvent effects, have been described (7,55). As one example, the conformations of the tetrapeptide Tyr -Gly -Gly -Phe, an enkephalin model, have been... 

---