Hydrodynamic chromatography porous wall

In Fl-FFF, the channel is created by placing a mylar spacer with the channel cut out between two porous frits. A membrane hlter of a specihc molecular weight cutoff is placed on one of the frits and acts as the accumulation wall to permit flow, without loss of particles. The applied force is then a perpendicular flow of the carrier solution across the porous frits. Fl-FFF is a versatile technique capable of separating macromolecules as small as roughly 1000 Da, in which case it is comparable to gel permeation (size exclusion) chromatography. However, Fl-FFF can also be applied to the separation of colloidal particles. In this case the hydrodynamic diameter of the colloidal particle is related to the retention volume, V by the equation... 

---