Hydrocarbons polycyclic aromatic, fluorescence enhancement

Sodiiun dodecylsulfate, cetyltrimethylam-monium chloride, sodium cholate, -cyclodextrin dansylated amino acids and polycyclic aromatic hydrocarbons > 45-fold 1% in water the greatest enhancement of fluorescence is that of sodium cholate on pyrene [263]... 

Likewise, the luminescence properties of many analytes can be altered in the presenoe of surfactant aggregates (4,7.,8.). Consequently, addition of micelle-forming surfactants (present either in the LC mobile phase or added post-column) can improve the sensitivity of fluorimetric LC detectors (49,482). Micellar spray reagents have been utilized to enhance the fluorescence densitometric detection of dansylamino acids or polycyclic aromatic hydrocarbons (483). The effect was observed for TLC performed on cellulose or polyamide stationary phases with the micellar spray reagent being either CTAC, SB-12, or NaC (483). More recently, use of nonionic Triton X-100 has been found to improve the HPLC detection of morphine by fluorescence determination after post-column derivatization (486) as well as improve the N-chlorination procedure for the detection of amines, amides, and related compounds on thin-layer chromatograms (488). 

In the case of fluorescence, that is, chemienergized by energy transfer, an energy acceptor is chosen that exhibits efficient fluorescence, for example, polycyclic aromatic hydrocarbons and, particularly, 9,10-disubstituted anthracene derivatives. Consequently, in the presence of such fluorescers (FI), the feeble direct chemiluminescence emission intensity is significantly enhanced. Such a phenomenon is commonly referred to as enhanced chemOuminescence (EC). 

Although CDs are generally used in the mobile phase as additives to improve separation, their application as extracting agent and sensitivity enhancer of the detection was also reported. Thus, a saturated solution of (3-CD was employed for the extraction of five polycyclic aromatic hydrocarbons (PAHs) from environmental samples. The application of (3-CD enhanced the efficacy of extraction and increased the sensitivity of fluorescence detection. The capacity of (3-CD to enhance the sensitivity of the chemi-luminscence detection of tetracyclines (oxytetracycline, tetracycline, and metacycline) has also been established. 

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