Hyaluronic acid oligosaccharides from

M sodium chloride oligosaccharides from hyaluronidase degradation of hyaluronic acid 89... 

Figure 9.14 Proteoglycan aggregate found in cartilage. C and N are C and N termini of the core protein. Long wavy lines represent chondroitin sulfate, whereas the short lines represent keratan sulfate. All are linkied to the core protein via the O-serine linkage, except for a few oligosaccharide chains near the N terminus of the core protein. LP is link protein, and HA is hyaluronic acid. (Reproduced by permission from Hascall V. Introduction. Functions of the Proteoglycans, Ciba Foundation Symposium 124. New York Wiley Sons, 1986, p. 2.)...

W4. Weissmann, B., Meyer, K., Sampson, P., and Linker, A., Isolation of oligosaccharides enzymatically produced from hyaluronic acid. J. Biol. Chem. 208, 417-429 (1954). 

Prehm (1983a) also explored the release of hyaluronate from the membranes and showed the phosphate esters prevented shedding of hyaluronate chains during active synthesis, but not under conditions where synthesis could not proceed. Oligosaccharides from hyaluronic acid would not displace it from membrane-bound synthetase, but it was released by trypsin or detergents. Hyaluronate chains appeared to be released randomly during synthesis and there was a continuous initiation of new chains even after two hours of incubation of membranes. 

The synthesis of hyaluronate lyase by a group A Streptococcus type 4 was enhanced by an infusion of trypsinized veal in the presence of oligosaccharides derived from hyaluronic acid. The purified enzyme (mol. wt. 5—7 x 10, pH optimum 6.0) gave a single band on polyacrylamide gel electrophoresis in the presence of... 

Mixed digests of hyaluronic acid and chondroitin sulfate (A or C) with testicular hyaluronidase yield, in addition to the degradation products from the parent polysaccharides, products of transglycosylation, consisting of hybrid oligosaccharides and containing repeating units from both polysaccharides (53b),... 

A method for preparing tritium-labelled oligosaccharides of hyaluronic acid has used the Wilzbach recoil technique the tritiated oligosaccharides are of potential use in studies of the mechanism of hyaluronidases. A four-fold helical form of hyaluronic acid has been detected using the data from fibre-... 

The purification of tritium-labelled oligosaccharides derived from hyaluronic acid has been improved, using a specific hyaluronidase. ... 

Oligosaccharides derived from hyaluronic acid and chondroitin 4-sulphate have been used as substrates to evaluate the glycoside hydrolase and glycosyl-transferase activities of the j8-D-2-acetamido-2-deoxyglucosidases A and B from bovine spleen. The glycoside hydrolase activity has a pH optimum of 4.5 and the glycosyltransferase has a pH optimum of 6.5. The B form exhibits equal hydrolase and transferase activities at pH 6.5, whereas the A form exhibits mainly hydrolase activity at this pH. 

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