Hyaluronic acid-coated surfaces

The above results suggest intimate association of proteins and proteoglycans in desmosomes and in surface coats of cells. A possible example of this kind of association is provided by studies of films made with various proportions of protein and proteoglycan. Mixtures of 2 parts gelatin and 1 part hyaluronic acid are much more resistant to diflFusion of various substances than other combinations of these ingredients (40). Perhaps intercellular discs represent mechanically dense and viscid complexes of similar nature. The disappearance of desmosomal components into reconstituted intercellular cement suggests that they have similar chemical components but different stoichiometry. 

In addition to PEG, certain polysaccharides, such as hyaluronic acid (HA) and dextran, have been used as low protein-adsorption, low cell adhesion surface coatings. Synthetic polymer surfactants consisting of poly(vinyl amine) with dextran and alkanoyl side chains, which mimicks the glycocalyx—negatively charged sugar layer outside cell membrane, are also shown to reduce protein adsorption to hydrophobic graphite surface. ... 

A report by Liu and coworkers described layer-by-layer (LBL) coatings that have been assembled on the inner surfaces of the microchip [90]. Natural polysaccharides, positively charged chitosan (CS), and negatively charged hyaluronic acid (HA) were multilayer-assembled onto the surface of a poly(ethylene terephthalate) (PET) microfluidic chip to form a microstructured and biocompatible network for enzyme immobilization. Trypsin was adsorbed in the multilayer membrane composed of CS/HA assembled multilayers. The resulting peptide analysis has been carried out by MALDI-TOF-MS. The maximum proteolytic velocity of the adsorbed trypsin was 600 mM/min/[ig, thousands of times faster than that in solution. BSA, MYO, and Cyt-c were used as model substrates for the tryptic digestion. The standard proteins were identifled at a low femtomole per analysis at a concentration of 0.5 ng/pl with the digestion time <5 s. This simple technique may offer a potential solution for low-level protein analysis. 

Tables Deposition of collagen-glycosaminoglycan conjugates on poly(octadecen-a/f-maleic anhydride)-coated substrates. Fibrillogenesis and immobilization of fibrillar collagen (1.2 mg/ml) was performed for 2 h at 37 ° C in the presence of heparin and hyaluronic acid (0.4, 1.2 and 5.0 mg/ml, respectively). Thickness of the layers was determined ellip-sometrically using the refractive index of 1.6035 for the dried collagen layer. The collagen amount was determined by amino acid-based HPLC analysis after acidic hydrolysis of surface-bound collagen...

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