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Hyaluronic acid, chromatography

Assay of hyaluronic acid by size exclusion chromatography... [Pg.237]

The same authors130 used gel chromatography on agarose for isolating the undegraded hyaluronic acid-protein complex from human synovial fluid. [Pg.46]

Lapcik, L., Jr., Chabreacek, P., and Staasko, A., Photodegradation of hyaluronic acid EPR and size exclusion chromatography study, Biopolymers, 31, 1429, 1991. [Pg.276]

Bull seminal-plasm a hyaluronidase has been purified 180-fold by affinity chromatography on concanavalin A-Sepharose , heparin-Sepharose , and chromatography on Sephadex G-200 and Sephacryl S-200. With hyaluronic acid as the substrate, the specific activity and turnover number of purified hyaluronidase were 3.63 /Umol min per mg (10400 National Formulary units mg of protein) and 214min (mol of product formed per mol of enzyme per min), respectively. Polyacrylamide gel electrophoresis indicated that the purified enzyme migrated as a single band at pH 4.3 and 5.3. Bull seminal-plasma hyaluronidase was markedly inhibited by hydroxylamine, phenyl-hydrazine, and semicarbazide. [Pg.513]

The chromatography of bovine serum albumin on gels of hyaluronic acid cross-linked with diepoxybutane has been investigated. A very sensitive spectrophotometric assay of hyaluronidase activity is based on the binding of a carbocyanine dye to the undegraded substrate-this results in a spectral shift... [Pg.479]

The protein core from keratan sulphate-containing proteoglycan of bovine nasal cartilage has been purified by affinity chromatography on matrix-bound hyaluronic acid. ... [Pg.324]

Gold, E. W. (1980). An interaction of albumin with hyaluronic acid and chondroitin sulfate A study of affinity chromatography and circular di-chioism. Biopolymers 9, 1407-1414. [Pg.377]

Yeung B, Marecak D. Molecular weight determination of hyaluronic acid by gel filtration chromatography coupled to matrix-assisted laser desorption ionization mass spectrometry. J Chromatogr A 1999 852 573-581. [Pg.224]

See other pages where Hyaluronic acid, chromatography is mentioned: [Pg.596]    [Pg.48]    [Pg.267]    [Pg.34]    [Pg.46]    [Pg.46]    [Pg.204]    [Pg.339]    [Pg.277]    [Pg.195]    [Pg.197]    [Pg.199]    [Pg.284]    [Pg.268]    [Pg.436]    [Pg.437]    [Pg.3]    [Pg.726]    [Pg.1482]    [Pg.39]    [Pg.350]    [Pg.74]    [Pg.419]    [Pg.326]    [Pg.453]    [Pg.251]    [Pg.290]    [Pg.299]   
See also in sourсe #XX -- [ Pg.369 ]



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