Human luteinizing hormone

Kudo, M., Osuga, Y., Kobilka, B. K., and Hsueh, A. J. W. (1996). Transmembrane regions V and VI of the human luteinizing hormone receptor are required for constitutive activation by a mutation in the third intracellular loop. / Biol. Chem. 271, 22470-22478. 

Muller T, Gromoll J, Simoni M. Absence of exon 10 of the human luteinizing hormone (LH) receptor impairs LH, but not human chorionic gonadotropin action. / Clin Endocrinol Metab 2003 88(5) 2242-9. 

Lee CY, Ryan RJ (1973) Interaction of ovarian receptors with human luteinizing hormone and human chorionic gonadotropin. Biochemistry 12 4609 1619 Marana R, Robertson DM, Suginami H, Diczfalusy E (1979) The assay of human follicle-stimulating hormone preparations the choice of a suitable standard. Acta Endocrin 92 599-614... 

Ivnitski and Rishpon [94] developed a one-step, separation-free, amperometric enzyme immunosensor, consisting of an antibody electrode that is low cost, disposable, and operates without washing or separation steps, using human luteinizing hormone (hLH) as a model analyte. Pencil lead was used as the working electrode as shown in Fig. 8.4. The immunosensor combines the... 

C3. Catt, K. J., Niall, H. D., Tregear, G. W., and Burger, H. G., Disc solid-phase radioimmunoassay of human luteinizing hormone. J. Clin. Endocrinol. Metab. 28, 121-126 (1968). 

K3. Kathan, R. H., Reichert, L. E., and Ryan, R. J., Comparison of the carbohydrate and amino acid composition of bovine, ovine and human luteinizing hormone. 

M7. Midgley, A. R., Radioimmunoassay A method for human chorionic gonadotropin and human luteinizing hormone. Endocrinology 79, 10-18 (1966). 

One major advantage of the glow-type emission obtained from dioxetane enzyme substrates is that one can easily record the emission on photographic film (in much the same way that emission is recorded) to obtain qualitative or semi-quantitative data. This technique has been described for a dry-format membrane-based assay for human luteinizing hormone (hLH), in which Polaroid 612 film (ASA 20,000) was used to record the intensity from unknown samples and from a set of standards (B18). A dioxetane-based ELISA method for human growth hormone (somatotropin) has been recently described. The chemiluminescent assay was reported to be superior to a similar fluorescence assay, and the detection limit for hGH was found to be 5 pM (A9). 

Figure 3.3. The structure of human luteinizing hormone-releasing hormone and its analogue goserelin.

Cho, N.B., Harada, M., Imaeda, T., Imada, T., Matsumoto, H., et al. (1998) Discovery of a novel, potent, and orally active nonpeptide antagonist of the human luteinizing hormone-releasing hormone (LHRH) receptor. J. Med. Chem. 41 4190-4195. 

Cellulose cyclic imidocarbonate can be coupled to bovine serum albumin and I-labelled human luteinizing hormone. A second antibody coupled to the cellulose derivative was used to separate antibody-bound and free hormones in radioimmunoassays of gonadotrophins in plasma and sera. 

G. De Placido, C. Alviggi, A. Perino, I. Strina, F. Lisi, A. Fasolino, R. De Palo, A. Ranieri, N. Colacurci, A. MoUo and on behalf of the Italian Collaborative Group on Recombinant Human Luteinizing Hormone, Hum. Reprod., 2005, 20, 390. 

Rizkallah, T., Taymor, M.L., Park, M., and Batt, R. (1%5) An inununoas-say method for human luteinizing hormone of pituitary ori. Journal of Clinical Endocrinology and Metabolism, 25,943-948. 

Veldhuis, J.D., and Iranmanesh, A. (2004) Pulsatile intravenous infusion of recombinant human luteinizing hormone under acute gonadotropinreleasing hormone receptor blockade reconstitutes testosterone secretion in young men. Journal of Clinical Endocrinology and Metabolism, 89,4474-4479. 

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