Human Insulin For Proteins of Higher Molecular Weight

HUMAN INSULIN FOR PROTEINS OF HIGHER MOLECULAR WEIGHT 

Materials Required Solution (1) Dissolve 10 mg of human insulin in 1 ml of the mobile phase, Solution (2) Dilute 100 pL of Solution (1) to 10 ml with the mobile-phase, and Solution (3) Dissolve 10 mg of human insulin EPCRS in 1 ml of the mobile-phase. 

Inject 50 pL of each solution. Adjust the sensitivity of the detector so that the height of the principal peak in the chromatogram obtained with solution (2) is 50 to 70% of full-scale deflection. In the chromatogram obtained with solution (1) the sum of the areas of any peaks eluting before the principal peak is not greater than the area of the principal peak in the chromatogram obtained with solution (2) (1.0%). 

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Human Insulin For proteins of higher molecular weight... 

Size Exclusion Chromatography (Chapter 31) has also been included as a means of analysis for substances that undergo separation more or less as per their molecular size, viz., insulin and human insulin—for proteins of higher molecular weight corticotrophin—for impurities of higher molecular weights and plasma-protein solution—for polymers and aggregates. 

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