HPLC-ICPMS arsenic

Figure 16.4. HPLC-ICPMS chromatogram of oyster tissue (1) arsenite (2) unknown (3) MMADPQ-arsenosugar (4) DMA (5) S04-arsenosugar (6) arsenate (7), (8) and (9) unknown (10) OH-arsenosugar (11) unknown (12) arsenobetaine (13) TMAO (14) arsenocholine (15) TMAs (courtesy of Dr Ute Kohlmeyer, GALAB Laboratories, Geesthacht).

J. Edmonds, Y. Shibata, R. Francesconi, R. Rippinggale, M. Morita, Arsenic transformations in short marine food chains studied by HPLC-ICPMS, Appl. Organomet. Chem., 11 (1997), 281-287. 

The concentrations of the three arsenicals (75-77) were determined in 37 marine organisms comprising algae, crustaceans, bivalves, fish and mammals by high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) [170]. All three organoarsenics, which occurred at pg/kg concentrations, were detected in 25, 23 and 17 of the 37 samples analyzed, respectively. The limits of detection were 2-3 pg/kg dry mass. The data illustrate that all three compounds are common minor constituents in practically all marine samples. 

The dimethylarsinoyl derivative of sulfated ribitol (see Fig. 2, compound 15) was isolated from the red alga Chondria crassicaulis (38). It had been observed as a major arsenical in C. crassicaulis by high performance liquid chroma-tography-inductively coupled plasma mass spectrometry (HPLC-ICPMS), and was initially reported as an unknown because it did not match any available standard (39). Subsequently, the compound was isolated and a chemical structure was proposed chiefly on nuclear magnetic resonance (NMR) data chemical synthesis of authentic material confirmed the proposed structure (38). This com-... 

Lipid arsenic compounds also occur in marine animals (34,110). The compounds originally present in the lipid fraction were subjected to base and/or acid hydrolysis, and the water-soluble products identified by HPLC-ICPMS. In this way, phosphatidylarsenocholine (see Fig. 2, compound 18) and a phosphatidyl-arsenosugar (see Fig. 2, compound 5) were identified in the digestive gland of lobster (34), and evidence was presented for the presence of lipids containing arsenocholine and dimethylated arsenic moieties in shark tissues (110). 

E. Schmeisser, W. Goessler, N. Kienzl, K. A. Francesconi, Volatile analytes formed from arsenosugars determination by HPLC-HG-ICPMS and implications for arsenic speciation analyses, Anal. Chem., 76 (2004), 418-423. 

Mazan, S., Cretier, G., Gilon, N., Mermet, J. M., Rocca, J. L. Porous graphitic carbon as stationary phase for LC-ICPMS separation of arsenic compounds in water. Anal Chem 2002, 74, 1281-1287. Bissen, M., Frimmel, F. H. Speciation of As(III), As(V), MMA and DMA in contaminated soil extracts by HPLC-ICP/MS. Fresenius J Anal Chem 2000, 367, 51-55. 

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