Horseradish peroxidase genes

Fujiyama K. Takemura H. Shinmyo A. Okada H. Takano M. (1990) Growth-stimulation of tobacco plant introduced the horseradish peroxidase gene prxCla / / Gene. V. 89. P. 163-169. 

Smith, A. T., Santama, N., Dacey, S., Edwards, M., Bray, R. C., Thomeley, R. N. F., and Burke, J. F., 1990, Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca and heme, J. Biol. Chem. 

Kemp and co-workers (K6) developed a colorimetric detection system that incorporates biotin into one (nested) primer and the sequence for a DNA-binding protein (e.g., the GCN4 gene from Saccharomyces cerevisiae) into the other primer. Amplified DNA is captured on an immobilized affinity reagent and the biotinylated product is detected with avidin-horseradish peroxidase and a chro-mogenic substrate. 

Because of the need for sensitivity, most labels for DNA analysis are enzymes or highly efficient fluor-ophores such as phycobiliproteins. The most commonly used enzyme labels are calf intestinal alkaline phosphatase and horseradish peroxidase (Table 2). Beta-galactosidase is used primarily for gene expression monitoring. Other enzymes such as glucose-6-phosphate dehydrogenase have not fovmd as wide utility as phosphatase and peroxidase. 

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