Hormones gold-labeled

The first report of this type was by Zavala and Brandon [48] who sectioned root tips at low temperature in order to try and prevent the redistribution of hormones. Their tissue sections were then probed with dihydrozeatin antibodies labelled with rhodamine. For electron microscopy, samples were freeze-substituted in ethanol or acetone and then embedded in polymers prior to sectioning and probing with colloidal gold-labelled antibodies. Although apparent specific labelling was reported in this work, it has been criticised [147] because dihydrozeatin is a relatively rare cytokinin, that had not been... 

Monoamine oxidase amperometric biosensor based on SPE were also modified with MWCNT by using the drop casting technique for the determination of antidepressants in model solutions and dosage forms. The authors used BSA protein which provided a matrix for the immobilization of the enzyme and protection of the enzyme activity when glutaraldehyde is used as a linker. Serafin et aZ. developed a label free dual immunosensor for the determination of human growth and prolactin hormones. The electrochemical immunosensor was based on CNT modify carbon SPE platform with the presence of poly(ethylene-dioxythiophene) (PEDOT) and gold nanoparticles. Again, the hybrid nano-material composite facilitated a proper immobilization of the antibody on the electrode matrix. 

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