Hormone receptor drug binding

Numerous interactions within cells and the body are characterized by the specific binding that occurs between two or more molecules. Examples include the binding of hormones with hormone receptors, drugs with enzymes or receptors, antibodies with antigens, and small solutes with transport proteins. The study of these interactions is important in determining the role they play in biological systems. Because of this, there have been many methods developed to characterize such reactions. One of these approaches is affinity chromatography. 

Mechanisms of drug action. To mediate a response the drug can bind to the desired therapeutic target or to other molecular targets such as G-protein-coupled receptors (GPCRs), ion channels, or transporters on the cell membrane, or to intracellular targets such as enzymes and nuclear hormone receptors. 

Bennett, J. P. Jr, Yamamura, H. L Neurotransmitter, hormone, or drug receptor binding methods, in Neurotransmitter Receptor Binding, ed. Yamamura, H. L, Enna, S. J., Kuhar, M. J., Raven Press, New York, 1985, pp 61-89. 

Hormone antagonism Application of drugs that bind to and inhibit the steroid hormone receptors or different types of releasing hormone receptors... 

Affinity chromatography can be applied to the isolation and purification of virtually all biological macromolecules. It has been used to purify nucleic acids, enzymes, transport proteins, antibodies, hormone receptor proteins, drug-binding proteins, neurotransmitter proteins, and many others. 

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