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Homogenous time-resolved fluorescence HTRF

Homogeneous Time Resolved Fluorescence (HTRF) (Cisbio International) is an assay based on the proximity of a lanthanide cryptate donor and a fluorescent acceptor molecule whose excitation wavelength overlaps that of the cryptate s emission. The utility of this technique is based on the time resolved fluorescence properties of lanthanides. Lanthanides are unique in the increased lifetime of their fluorescence decay relative to other atoms, so a delay in collection of the emission intensity removes the background from other fluorescent molecules. An example of the HTRF assay is a generic protein-protein interaction assay shown in Fig. 2. [Pg.39]

Primary Homogeneous Time-Resolved Fluorescence (HTRF) HTS Assay.84... [Pg.83]

PRIMARY HOMOGENEOUS TIME-RESOLVED FLUORESCENCE (HTRF) HTS ASSAY... [Pg.84]

Inherent fluorescence in natural product samples rules out the use of frank fluorescence as an assay readout. In the early 1990s, the development of homogeneous time-resolved fluorescence (HTRF) allowed the use of fluorescence with such samples (Kolb, Yamanaka, and Manly, 1996 Kolb and Manly, 1997 Bazin, Trinquet, and Mathis, 2002 Mathis, 1999). This technique... [Pg.217]

Because of the very stringent reliability requirements for diagnostic applications, the only homogeneous techniques that have survived in this field are the fluorescence polarization (FP) methods originally developed by AbbotE and the homogeneous time resolved fluorescence (HTRF) and TRACE approaches developed later by CIS bio international. [Pg.234]

An example of a recent development in assay technology that combines speed, sensitivity and safety is homogeneous time resolved fluorescence (HTRF , Packard). HTRF has the inherent sensitivity associated with fluorescence, but is designed to eliminate or compensate for problems such as high background, color interference and fluorescence quenching often found in biological assays. [Pg.112]

HTRF (homogeneous time-resolved fluorescence) LANCE (lanthanide chelate excitation) Cisbio International PerkinElmer Life Sciences Lanthanide TR-FRET using Eu3+ cryptate/chelate-donor fluorophore and cross-linked aUophycocyanin-acceptor fluorophore... [Pg.88]

The homogeneous TRF assay formats HTRF ( homogeneous time-resolved fluorescence ) by GisBio International [115, 120, 136, 137] and LANGE ( lantha-... [Pg.639]

HTRF/LANCE - homogeneous time-resolved fluorescence/lanthanide cryptate excitation SPA - scintillation proximity assay - homogeneous assay which detects radioisotopes in close proximity to a solid scintillant ... [Pg.964]

HTRF is a homogeneous technology which combines an FRET process with time-resolved fluorescence detection to probe biomolecular interactions. This combination is made possible through the use of a long lifetime fluorescent FRET donor, a lanthanide cryptate. Cryptates are formed by the inclusion of a rare earth ion e.g. [Pg.241]

See other pages where Homogenous time-resolved fluorescence HTRF is mentioned: [Pg.377]    [Pg.11]    [Pg.7]    [Pg.167]    [Pg.326]    [Pg.268]    [Pg.61]    [Pg.361]    [Pg.118]    [Pg.424]    [Pg.66]    [Pg.377]    [Pg.11]    [Pg.7]    [Pg.167]    [Pg.326]    [Pg.268]    [Pg.61]    [Pg.361]    [Pg.118]    [Pg.424]    [Pg.66]    [Pg.3]    [Pg.200]    [Pg.35]    [Pg.169]    [Pg.48]    [Pg.326]    [Pg.47]    [Pg.89]    [Pg.112]   
See also in sourсe #XX -- [ Pg.424 ]



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Homogeneous time resolved fluorescence

Homogeneous time resolved fluorescence HTRF)

Homogeneous time resolved fluorescence HTRF)

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