Histamine and Serotonin Detection

FIA with amperometric detection was also used to assay these two compounds at diamond electrodes. A detection limit of 0.5 pM (S/N = 13.8) was found for histamine, while a low, 10 nM detection limit was observed for serotonin. Diamond exhibited a linear dynamic range from 10 nM (S/N = 18.1) to 100 pM for serotonin and from 0.5 to 100 pM for 

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Direct Electron Transfer to Heme Peptide and Peroxidase 

Different results were obtained for the peroxidase-modified electrodes. For this biomolecule, the edge plane of pyrolytic graphite and glassy carbon produced larger cathodic currents than did either the 

Clearly, the electrode reaction mechanism and kinetics for these two biomolecules are different at diamond electrodes than they are at other SP -bonded carbon electrodes. Further research is needed to understand these differences. This work, however, reveals that direct electron exchange can occur between biomolecules, such as peptides and proteins, and diamond electrodes and that the response can be influenced by the surface chemistry. 

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