Hinoki cypress

Figure 23. Curves of heat of wetting W (log scale) for Hinoki cypress and for cotton against the moisture content (48).

Fig. 2. Somatic embryogenesis in Hinoki cypress. A Collected open-pollinated cones. B Proliferation of induced embiyogenic tissue on medium containing auxin and cytokinin. C Embryogenic cells. D-F Different developmental maturation stages of somatic embryos. G Production of somatic embryos. H Germination of somatic embryos. I Plantlets growing in vitro. J Acclimatized plants derived from somatic embryos. K-L Somatic plants growing out in the field. Bars 1mm (C-F), 1cm (A-B, G-J), Im (K-L)...

Table 4. Somatic embryo production, germination and plant conversion for 34 cell lines of Hinoki cypress...

Maruyama, E., Ishii, K., and Hosoi, Y. (2005) Efficient plant regeneration of Hinoki cypress Chamaecyparis obtusa) via somatic embryogenesis. J. For. Res., 10 73-77. 

For products such as soaps and shampoos containing cypress oil extract, Hinoki-tiol has been developed. Hinokitiol has an antibacterial or bactericidal effect, but it is volatile and rapidly decomposes by oxidation. CyDs can stabilize Hinokitiol. 

In this chapter we describe a stable and efficient plant regeneration system for the Hinoki and Sawara cypress via somatic embryogenesis. The initiation of embryogenic cultures (EC), their maintenance and proliferation, maturation of somatic embryos, germination... 

Immature open-pollinated cones of the Hinoki and Sawara cypress (Fig. lA and Fig. 2A) were collected in June and July from plus mother trees. The collected cones were subsequently disinfected by 1 min immersion in 99.5% ethanol and dried in the laminar flow cabinet before dissection. The excised seeds were disinfected with 1% (w/v available chlorine) sodium hypochlorite solution for 15 min and then rinsed five times with sterile distilled water. After the seed coats had been removed, the megagametophytes containing immature zygotic embryos were used as explants for ECI initiation. 

Regenerated emblings of Hinoki (Fig. 21) and Sawara cypress (Fig. IL) were transferred to 300 ml flasks containing 100 mL of fresh medium (same composition used for the germination and conversion but with 30 gL i sucrose and 5 gL-i AC) and kept under the... 

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