High voltage electron

Li C. and Cooper S.L., Direct observation of micromorphology of polyether pol3mrethane using high voltage electron microscopy, Polymer, 31, 3, 1990. 

Somljo It all depends on the fixation method. We use osmium ferricyanide to selectively infiltrate the SR. If we use intermediate high-voltage electron microscopy, we can look at thicker specimens, and this technique provides more extensive views than obtained from the usual thin sections. This is the same information we get when we infiltrate the SR with Fluo-3. These pictures are pretty reliable. Furthermore, if you want to confirm without chemical fixation, methods such as rapid freezing are available. All these techniques give the same pictures, which vary according to the smooth muscle type. 

Marty F. High voltage electron microscopy of membrane interactions in wheat. JHistochem Cytochem 1980 28 1129-1132. 

Hawes CR. Applications of high voltage electron microscopy to botanical ultrastructure. Micron 1981 12 227-257. 

Other more exotic mechanisms include chemical reactions in gases, high-voltage electron beam pumping of gases, and direct current injection across the junction of a semiconductor laser. 

Evidence from conventional and high-voltage electron microscopy and three-dimensional imaging./. Struct. Biol. 117, 24-35. 

Figure 5. High-voltage electron micrographs and x-ray spectra of microparticles collected at Whiteface Mountain in 1983. Scale bars equal 0.5 pm. (a) y-Fe203 sphere collected 28 July II. (b) y-Fe203 sphere cluster collected 28 July IV. (c) Mullite sphere collected 28 July IV. (d). Mixed-element sphere collected 28 July III.

Invited 7. Young-Min Kim, Jin-Kyu Kim and Youn-Joong Kim (Korea Basic Science Institute) Electron Spectroscopy by Energy-Filtered High Voltage Electron Microscopy... 

Figure 4. Strength of cotton print clothes thermally degraded by heating at 168°C for various times. The strength of fabrics degraded by hydrolyzing 16 hr in 40% sulfuric acid at room temperature and by high-voltage electron irradiation at doses of 50 and 100 Mrad are also indicated.

Figure 6. Reflectance as a function of wavelength of contemporary cotton print cloth degraded by a 100-Mrad dose of high-voltage electrons before and after Turnbults Blue test for carboxyl groups...

Figure 13. Fracture tip surface of a typical cotton fiber degraded by exposure to a 100-Mraa dose of high-voltage electrons...

A tissue section cut from a frozen specimen in this situation, ice is the supporting matrix. See Yamada, E. and Watanabe, H., High voltage electron microscopy of critical-point dried cryosection, J. Electron Microsc. 26 (SuppL), 339-342, 1977 Maddox, P.H., Tay, S.K., and Jenkins, D., A new fixed cryosection technique for the simultaneous immuuocytochem-ical demoustratiou of T6 and SlOO antigens, Histochem. J. 19, 35-38,... 

Gandais, M., Strunk, H. (1983). Microstructure of plastically deformed sanidine (K-feldspar). In Proc. 7th Int. Corf, on High Voltage Electron Microscopy, Berkeley, edited by R. M. Fisher, R. Gronsky, K. H. Westmacott, pp. 353-8. Berkeley (Laboratory), CA, and ENTIS, Springfield, VA. 

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