High-throughput screening solubility

Bevan, C. D., Uoyd, R. S. A high-throughput screening method for the determination of aqueous drug solubility using laser nephelometry in microtiter plates. Anal. Chem. 2000, 72,1781-1787. 

Huuskonen, J. Estimation of aqueous solubility in drug design. Comb. Chem. High-Throughput Screen. 2001, 4, 311-316. 

Fligge, T.A. and Schuler, A. 2006. Integration of a rapid automated solubility classification into early validation of hits obtained by high throughput screening. J. Pharm. Biomed. Anal. 42 449. 

Onofrey, T. and Kazan, G. Performance and Correlation of a 96-Well High throughput Screening Method to Determine Aqueous Drug Solubility, Application Note (Billerica, MA Millipore Corp., 2003). 

At the end of this Chapter, looking at the exceptional variety of water-soluble ligands and the pace with which newer and newer compounds are synthetized it is safe to state that every aqueous reaction may find its perfect catalyst - at least the ligands are out there already. It seems that high-throughput screening could benefit aqueous organometallic catalysis, too. 

Chambers, S. R, Austen, D. A., Fulghum, J. R. and Kim, W. M. (2004). High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells. Protein Expr. Purif. 36, 40-47. 

Almost any solvent is possible for a recrystallization. Table 13.1 shows some of the most frequently used solvents in the pharmaceutical industry. Mixtures of solvents, binary or even ternary, are also common. The burden of checking many solvents and solvent mixtures for use in recrystallizations can be mitigated with automated high-throughput screening tools. A drug s solubility is tested in each solvent and reasonable solvent mixture at various... 

High expression levels of soluble and active Mur enzymes in E. coli enabled the rapid purification of large amounts of enzyme for the high-throughput screen. Approximately 120 mg of purified Mur enzymes could be purified from each liter of expression culture. 

A high-throughput screen for S. pneumoniae MurD was developed that did not require radioactivity. The high expression levels of soluble active Mur enzymes in E. coli allowed large quantities of enzymes to be purified rapidly. The chemical... 

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