High-resolution spectrometry, drug instrumentation

Accurate mass measurements are often used for the identification or confirmation of the molecular formulas of drug metabolites. The analytical utility of high resolution mass spectrometry began with the introduction of double-focusing magnetic sector instruments, which could routinely achieve... 

Endogenous interferences and MALDI matrix cluster can complicate the analysis of xenobiotic drug compounds and their metabolites in MSI. The use of hyphenated techniques (either MS" or IMS) or high-resolution mass spectrometry can serve to increase analyte specificity. Although instrument... 

Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) instrumentation offers excellent sensitivity, accuracy (<1 ppm), and high mass resolution (>1,000,000) (Table 10.2). However, because of being too expensive, difficult to use, and not compatible with conventional HPLC columns and flow rates, FTMS has not been frequently used in pharmaceutical research. This changed with an introduction of a hybrid instrument consisting of a linear ion-trap mass spectrometer compatible with LC and an ion-cyclotron-resonance (ICR) detector. Such a hybrid instrument is compatible with conventional HPLC and allows for acquisition of accurate mass data-dependent MS" spectra. Sanders et al. [128] recently reviewed the utility of hybrid LTQ-FTMS for drug metabolism and metabonomics applications while Brown et al. [129] reviewed the metabolomics applications of FT-ICR-MS. 

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