Electrophoresis high-resolution

Polyacrylamide shows many advantages over starch gel as a medium for high resolution electrophoresis and because of its synthetic nature its pore size can be more easily controlled. The gel is formed by the polymerization of the two monomers, acrylamide and a cross-linking agent, N, iV-methylene-bis-acrylamide (Figure 3.26). The proportion of the two monomers and not their total concentration is the major factor in determining the pore size, the latter having more effect on the elasticity and... 

Hopwood JJ, Harrison JR (1982) High-resolution electrophoresis of urinary glycosaminoglycans an improved screening test for the mucopolysaccharidoses. Anal Biochem 119 120-127... 

Maizel introduced the use of sodium dodecylsulfate (SDS) for high-resolution electrophoresis of protein mixtures. 

D. F. Keren, High Resolution Electrophoresis and Immunofixation, Butterworths, London, 1987. 

Keren DF. High-resolution electrophoresis and immunofix-ation. Techniques and interpretation. Oxford Butter-worth-Heinemann, 1994. 

Keren DE High Resolution Electrophoresis and Immunofixation Techniques. Boston Butterworth-Heineman, 1994. 

Capillary electrophoresis High-speed, high-resolution electrophoresis performed in capillary tubes or in microchips. Carbonate error A systematic error caused by absorption of carbon dioxide by standard solutions of base that will be used in the titration of weak acids. 

From the model in Scheme 10, a further prediction can be made that an even weaker oxidant than RudlDOH should be reduced efficiently in the presence of DNA with no detectable damage to the nucleic acid—i.e., only the kx pathway is operative. This prediction is confirmed by the results on Os(tpy)(bpy)0. It has been shown by X-ray crystallography that Os(tpy)(bpy)OH2 is structurally identical to the ruthenium analogue (Fig. 16), so the only difference in the complexes is the weaker oxidizing power of the osmium complex. Nevertheless, Os(tpy)(bpy)0 does not damage DNA as assessed by high-resolution electrophoresis, even though it is reduced efficiently in DNA 186). Thus, reduction of Os(tpy)(bpy)0 occurs via the kx pathway, and it is possible to use the time dependence of osmium reduction to measure kx directly. These studies show that self-inactivation of Os(tpy)(bpy)0 is catalyzed by DNA (Fig. 17). The extent of catalysis is a function of the square of the concentration of osmium bound to DNA calculated... 

Abate, O., R. Zanatta, T. Mahsano, and U. Dotta. 2000. Canine serum proteins using high-resolution electrophoresis (HRE). Veterinary Journal 159 154-160. 

Method The method used is DNA extraction from blood, bone marrow or tissue (fresh or formalin-fixed paraffin-embedded tissues), followed by PCR (multiplex PCR) using specific primers. To provide a better discrimination of the monoclonal bands it is recommended to run out the PCR product by polyacrylamide gel electrophoresis. High-resolution electrophoresis is essential to analyze small fragments of the rearranged of TCR y chain with slight variations. A heteroduplex assay for the analysis of the PCR product improves the results and minimizes the possibility of false-positive and false-negative results due to improper primer annealing. 

Anderson, N. G., and Caton, J. E. (1973), High resolution electrophoresis Immuno-subtraction. In preparation. 

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