High-performance size exclusion chromatography, experimental

In recent years, aqueous size exclusion chromatography applied In analytical studies of blomacromolecular systems has rapidly advanced In both theoretical and experimental aspects. The appearance of high performance gel chromatography materials has enabled us to separate proteins, nucleic acids and other blopolymer assemblies with high resolution. 

The determination of the molecular weight of nanoparticles is performed by gel permeation chromatography (GPC). The experimental setup consists of a high performance liquid chromatography system with a size exclusion column and a refractive index detector. The nanoparticles are usually freeze-dried and dissolved in tetrahydrofuran for analysis on the system. Poly(styrene) or poly(methylmethacrylate) standards are used to calibrate the column, to enable the determination of number average molecular weight (Mn), as in... 

High performance liquid chromatography (HPLC) has been widely used for the separation and fractionation of many substances due to the short experimental time, high resolution and excellent reproducibility. There are three types of HPLC system used for analysis reversed phase chromatography, ion exchange chromatography and size exclusion or gel permeation chromatography. 

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