High performance liquid chromatography theory

A.M. Krstulovic and P.R. Brown, Reversed-Phase High Performance Liquid Chromatography Theory, Practice and Biomedical Applications, Wiley, New York, 1982. 

R. Kaliszan, P. Wiczling and M.J. Markuszewski, pH gradient high-performance liquid chromatography theory and application. J. Chromatogr.A 1060 (2004) 165-175. 

Krstulovic A M, Brown P R 1982 Reversed phase high-performance liquid chromatography theory, practice, and biomedical applications. John Wiley New York, 296 pp... 

Colin, H. and Krstulovic, A. M., The theory of retention in reversed-phase high-performance liquid chromatography, in Liquid Chromatography in Pharmaceutical Development, Wainer, I. W., Ed., Aster Publishing, 1985, 171. 

High performance liquid chromatography is a technique that has arisen from the application to liquid chromatography (lc) of theories and instrumentation that were originally developed for gas chromatography (gc). 

It was known from gas chromatographic theory that efficiency could be improved if the particle size of the stationary phase materials used in lc could be reduced. High performance liquid chromatography developed steadily during the late 1960s as these high efficiency materials were produced, and as improvements in instrumentation allowed the full potential of these materials to be realised. As hplc has developed, the particle size of the stationary phase used has... 

For the pharmaceutical scientist, understanding the theory and application of the equipment is usually not sufficient there is the matter of compliance. The qualification of CE is similar to that of other instruments. Installation qualification (IQ), operational qualification (OQ), and performance qualification (PQ) apply in much the same way as high-performance liquid chromatography (HPEC). This chapter details the different parts of the modern CE instrument, equipment-related issues and troubleshooting, instrument qualification, and the future of the CE instrument. 

The process of analyte retention in high-performance liquid chromatography (HPLC) involves many different aspects of molecular behavior and interactions in condensed media in a dynamic interfacial system. Molecular diffusion in the eluent flow with complex flow dynamics in a bimodal porous space is only one of many complex processes responsible for broadening of the chromatographic zone. Dynamic transfer of the analyte molecules between mobile phase and adsorbent surface in the presence of secondary equilibria effects is also only part of the processes responsible for the analyte retention on the column. These processes just outline a complex picture that chromatographic theory should be able to describe. 

Pyka and Sliwiok separated a-tocopherols, p-tocopherols, y-tocopherols, and 8-tocopherols by normal-phase high-performance liquid chromatography (NP-HPLC) and RP-Cig-HPLC. The selected topological indices based on connectivity (M, x ")> distance matrix W, MTI), and information theory (Zac, Tag) were... 

Weber, S. G., Carr, P. W. The Theory of the Dynamics of Liquid Chromatography in High Performance Liquid Chromatography, Brown, P. R., Hartwiclc, R. A. (Eds.), Wiley, New York, 1989. 

J.H. Knox Theory of HPLC, Part II Solute Interactions with the Mobile Phase and Stationary Phases in Liquid Chromatography . In C.F. Simpson (ed.), Practical High Performance Liquid Chromatography. Heyden and Son, Chichester 1976. 

---