High performance liquid chromatography pore size

Prior to high-performance liquid chromatography (HPFC), samples were filtered through a 0.2-gm pore size mixed cellulose ester filter (Schleicher Schuell, Dassel, Germany). Buffer components such as acetic acid, citric acid, maleic acid, and succinic acid were separated on an Aminex HPX-87H (Bio-Rad, Hercules, CA) organic acid column at 65°C using a... 

The apparatus used for SEC is relatively simple isocratic high-performance liquid chromatography (HPLC) equipment, as shown in Figure 8. The principal components are a precision high-pressure solvent delivery system, detector, and a column with particles having appropriate pore size. Detection is conveniently accomplished with a UV photometer or spectro-... 

The suspension of an SPM sample in 100mL of CH2CI2 is sonicated for 15 min and filtered with a membrane that has a 0.1-pm pore size, the filtrate is evaporated, and the residue is resolved in acetonitrile [24]. The concentration of the chemicals in the SPM extracts is determined using a multicolumn high-performance liquid chromatography (HPLC)/spectrofluorometer/computer system. According to EQ = S([PAH]iEFi +. ..+ [PAH] EF ), the chemically derived induction equivalent (EQ) is calculated to standardize the biological effect of the chemical on the EROD capacity of the Flep G2 cells [25]. 

While we have only focused here on applications for DNA electrophoresis, colloidal crystals have also been used as a stationary phase for separating hydrophobic dyes and proteins [40]. The arrays may prove especially interesting for electrophoretic protein separations, since the pore size provides a steric constraint, no pressure drop is required for transport through the small pores and the bead surfaces serve as a substrate for reversed-phase adsorption. These arrays thus offer a tunable variety of separation mechanisms. Indeed, proteins with similar hydrophobic groups, which cannot be separated by high performance liquid chromatography (HPLC) due to their similar adsorption properties, are rapidly separated by charge in colloidal arrays [40]. 

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