High performance liquid chromatography optimisation

A number of sequential analyses are carried out automatically with the mobile phase composition being adjusted between analyses as determined by the optimisation procedure. Typically five to 10 analyses are required for optimisation. 

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Aguilar, M. I., Hodder, A. N., and Hearn, M. T. W., High-performance liquid chromatography of amino acids, peptides, and proteins. LXV. Studies on the optimisation of the reversed-phase gradient elution of polypeptides. Evaluation of retention relationships with (3-endorphin-related polypeptides, /. Chromatogr., 327, 115, 1985. 

K. Jones, Optimisation procedure for the silanisation of silicas for reversed-phase high-performance liquid chromatography. I. Elimination of nonsignificant variables, J. Chromatogr., 392 (1987) 1-10. 

K. Jones, Process scale high-performance liquid chromatography. Part I An optimisation procedure to maximise column efficiency, Chromatographia, 25 (1988), 437-442. 

Acetonitrile-silver nitrate extracts of soil, partitioned with methylene chloride and cleaned up on silica gel, have been used in the high-performance liquid chromatographic determination of benfuracarb and carbofuran herbicides [204], McGarevy et al. [336] and Lin and Cooper [336] used optimised isocratic high-performance liquid chromatography with UV detection to determine Aldicarb and its metabolites in soil. 

High performance liquid chromatography (HPLC) is used routinely in phytochemistry to "pilot" the preparative isolation of natural products (optimisation of the experimental conditions, checking of the different fractions throughout the separation) and to control the final purity of the isolated compounds [2]. HPLC is the most well fitted technique for an efficient separation of crude plant extracts and can be coupled with different spectroscopic detection methods. 

Optimisation of sample preparation for the determination of trans-resveratrol and other polyphenolic compounds in wines by high performance liquid chromatography. Anal. Chim. Acta. 428, 245-253. 

The second group of recently developed ionic liquids is often referred to as task specific ionic liquids in the literature [15]. These ionic liquids are designed and optimised for the best performance in high-value-added applications. Functionalised [16], fluorinated [17], deuterated [18] and chiral ionic liquids [19] are expected to play a future role as special solvents for sophisticated synthetic applications, analytical tools (stationary or mobile phases for chromatography, matrixes for MS etc.), sensors and special electrolytes. 

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