High-performance liquid chromatography column technology

SynChropak GPC supports were introduced in 1978 as the first commercial columns for high-performance liquid chromatography of proteins. SynChropak GPC columns were based on research developed by Fred Regnier and coworkers in 1976 (1,2). The first columns were only available in 10-yu,m particles with a 100-A pore diameter, but as silica technology advanced, the range of available pore diameters increased and 5-yu,m particle diameters became available. SynChropak GPC and CATSEC occasionally were prepared on larger particles on a custom basis, but generally these products have been intended for analytical applications. 

CE Goewie, P Kwakman, RW Frei, UATh Brinkman, W Maasfeld, T Seshadri, A Kettrup. Pre-column technology in high-performance liquid chromatography for the determination of phenylurea herbicides in water in the presence of their anilines. J Chromatogr 284 73-86, 1984. 

Early in the development of capillary electrophoresis, it was noted that the successful detection of separated sample components present within the narrow confines of these capillary tubes posed a major challenge (2)- In response to this challenge, much research has been directed toward the development of sensitive and selective detectors for capillary electrophoresis. CE detector technology has been largely borrowed from the field of high-performance liquid chromatography (HPLC), especially from micro-column HPLC. 

Quantitation of 1,4BD by high-performance liquid chromatography (HPLC) with photo-diode-array detection was described by Duer et al. (2001). The 1,4BD was extracted from the biological matrix with acetonitrile and derivatized with 3.5-dinitrobenzoyl chloride. This method has a linear range from 3 to 250 rng L with a limit of quantitation of 10 rng L . Also, as was mentioned previously, the United Chemical Technologies GHB SPE column method has been modified to include 1,4BD analysis (Kraner et al., 2000). 

With the technological evolution during the last 15 years high efficiency column materials have appeared, and with them the development of high-performance liquid chromatography. 

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