High performance liquid chromatography-circular dichroism

Synovec, R.E. Yeung, E.S. Fluorescence detected circular dichroism as a detection principle in high-performance liquid chromatography. J. Chromatogr. 1986, 368, 85-93. 

Westwood, S.A. Games, D.E. Sheen, L. Use of circular dichroism as a high-performance liquid chromatography detector. Chromatography 1981, 204, 103-107. 

There are many other useful analytical methods. Chromatographic methods such as gas chromatography (GC) and high-performance liquid chromatography (HPLC) are used daily for identification and estimation of the purity of a synthetic product. Chiroptical methods, such as circular dichroism (CD) spectroscopy, are also important especially in studying the relationships between absolute configuration and bioactivity of biofunctional molecules. In later chapters I will give some examples of application of CD spectroscopy in enantioselective synthesis. 

Ac AIBN 9-BBN Bn Boc Bu Bz CAN Cbz CD CSA DABCO DAST DBN DBU DCC DDQ DEAD DHP DIAD DIBAL-H DMAP DME DMF DMP DMSO DNB EE Ee Eq Et Fmoc GLC HLADH HMDS HMPA HOBt HPLC Im acetyl 2,2/-azobisisobutyronitrile 9-borabicyclo[3.3.1]nonane benzyl f-butoxycarbonyl butyl benzoyl ceric ammonium nitrate benzyloxycarbonyl circular dichroism camphorsulfonic acid 1.4- diazabicyclo[2.2.2]octane A,A-Diethylaminosulfur trifluoride 1.5- diazabicyclo[4.3.0]non-5-ene l,8-diazabicyclo[5.4.0]undec-7-ene A,A -dicyclohexylcarbodiimide 2.3- dichloro-5,6-dicyano-1,4-benzoquinone diethyl azodicarboxylate 3.4- dihydro-2//-pyrane diisopropyl azodicarboxylate diisobutylaluminum hydride 4-A,A -dimethylaminopyridine 1,2-dimethoxyethane A,A -dimethylformamide Dess -Martin periodinane [1,1,1 -tris(acetyloxy)-1,1 -dihydro-1,2-benziodoxol-3-( IH) -one] dimethyl sulfoxide 3.5- dinitrobenzoyl 2-ethoxyethyl enantiomeric excess molar equivalent ethyl 9-fluorenylmethoxycarbonyl gas-liquid chromatography horse liver alcohol dehydrogenase 1,1,1,3,3,3 -hexamethyldisilazane hexamethylphosphoric triamide 1 -hydroxybenzotriazole high-performance liquid chromatography 1-imidazolyl or imidazole... 

Abbreviations BChl - bacteriochlorophyll CD - circular dichroism, EPR - electron paramagnetic resonance HPLC -high performance liquid chromatography NMR - nuclear magnetic resonance P-870 - primary donor RC - reaction center... 

To investigate the stereochemistry of pentacoordinated spirophosphoranes with chiral chelate ligands such as amino acids, the diastereoi-somers could be differentiated by reverse-phase high-performance liquid chromatography (HPLC), solution or solid-state circular dichroism (CD),... 

The analysis of reaction enantioselectivity for asymmetric reductions is key for the process development and an interesting combined use of high-performance liquid chromatography and circular dichroism has led to an efficient procedure for alcohol dehydrogenase-catalyzed asymmetric reduction of l-phenyl-2-propyn-3-trimethyl-silyl-l-on [133]. 

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