Fluorescence detected circular dichroism

Meadows F, Narayanan N, Patonay G (2000) Determination of protein-dye association by near infrared fluorescence-detected circular dichroism. Talanta 50 1149-1155... 

Maupin, C. L. Riehl, J. P. Circularly polarized luminescence and fluorescence detected circular dichroism. In Encyclopedia of Spectroscopy and Spectrometry, Lindon, J. C. Trantner, G. E. Holmes, J. L., Eds. Academic Press, 2000 pp 319-326. 

Chiral isocyanides such as 198 have also been used for the synthesis of chiral 4,5-disubstituted oxazoles such as 199, which are potentially useful in fluorescence-detected circular dichroism for on-column capillary electrophoresis (Equation 12) <1996JOC8750>. 

Synovec, R.E. Yeung, E.S. Fluorescence detected circular dichroism as a detection principle in high-performance liquid chromatography. J. Chromatogr. 1986, 368, 85-93. 

Thomas, M.P. Patonay, G. Warner, I.M. Fluorescence-detected circular dichroism studies of serum albumins. Anal. Biochem. 1987,164, 466-473. 

Fluorescence-detected circular dichroism (FDCD) is a chiroptical technique in which the spectrum is obtained by measuring the difference in total luminescence obtained after the sample is excited by left- and right-circularly polarized light. For the FDCD spectrum of a given molecular species to match its CD spectrum, the luminescence excitation spectrum must be identical to the absorption spectrum. 

The large environmental dependence of the ll-(3-hexyl-l-indolyl)undecyltri-methylammonium bromide fluorescence lifetime and fluorescence spectrum has led to its continued use as a fluorescence probe for micellar systems.76 Fluorescence-detected circular dichroism, a technique which appears to have considerable potential, has been applied to the determination of the c.d. spectra of L-tryptophan and L-cystine and two macromolecular systems.78... 

Since polypeptides are chiral polymers, the chromophore arrangement shows some chirality. Therefore, additional information on the chromophore arrangement in the ground state, as well as in the excited state, may be obtained from chiroptical spectroscopy, such as circular dichroism (CD), circularly polarized fluorescence (CPF) [43], and fluorescence-detected circular dichroism (FDCD) [44). 

Use of polarized light to excite fluorescence, and measurement of the state of polarization of the emitted light introduce another set of measurable parameters that can characterize structures and dynamics of molecules. The anisotropy of the polarization of fluoresence after excitation by linearly polarized light provides the rotational diffusion coefficient, or rotational correlation time, of the fluorophore. When there is fluorescence energy transfer, analysis of the anisotropy of both donor and acceptor can reveal the relative orientation, and the relative motion. Measurement of fluorescence after excitation by circularly polarized light provides the fluorescence-detected circular dichroism. This measurement characterizes the chiral environment of the ground state of the fluorophore. If the circular polarization of the fluorescence is measured, the circularly polarized luminescence is obtained. This measurement characterizes the chirality of the excited state. 

Application of fluorescence-detected circular dichroism (FDCD) to the determination of the major pterin (L-monapterin) from Escherichia coli has been published <01H(54)283>. Identification of (6/ )-5,6,7,8-tetrahydro-D-monapterin as the native pleridine in Tetrahymena pyriformis has been reported <01HCA918>. A novel modified pterin has been isolated from... 

Magnetic circular dichroism, a more recent development, has been used mainly in the study of haem proteins and has been reviewed recently as has been fluorescence-detected circular dichroism (FDCD). 

A new method which is more specific than circular dichroism is fluorescence detected circular dichroism (FDCD). In this method the intensity of light emitted by a fluorescent chromophore excited by left and right circularly polarized light is used to measure the circular dichroism. The FDCD method thus gives information about the conformation of a polymer in the immediate neighborhood of fluorescent groups. 

Fig. 11. Experimental arrangement for measuring fluorescence detected circular dichroism.

Fig. 12. An illustration of how fluorescence detected circular dichroism (FDCD) allows the measurement of the CD of a fluorescent chromophore in the presence of a non-fluorescent, but optically active chromophore. The usual CD measures the sum of the CD of L-cystine and L-tryptophan. The FDCD measures only the fluorescent L-tryptophan. (Reprinted from D.H. Turner, M.F. Maestre, and I. Tinoco, Jr. J. Am. Chem. Soc. 96, 4340 (1974).)...

Circularly Polarized Luminescence and Fluorescence Detected Circular Dichroism... 

Biochemical Applications of Fluorescence Spectroscopy Biomacromolecular Applications of Circular Dichroism and ORD Biomacromolecular Applications of UV-Visible Absorption Spectroscopy Chemical Reactions Studied By Electronic Spectroscopy Circularly Polarized Luminescence and Fluorescence Detected Circular Dichroism Dyes and Indicators, Use of UV-Visible Absorption Spectroscopy... 

Circularly Polarized Luminescence and Fluorescence Detected Circular Dichroism Induced Circular Dichr-oism Magnetic Circular Dichroism, Theory ORD and Polarimetry Instruments Rotational Spectroscopy, Theory Vibrational CD Spectrometers Vibrational CD, Applications Vibrational CD, Theory. 

CIRCULARLY POLARIZED LUMINESCENCE AND FLUORESCENCE DETECTED CIRCULAR DICHROISM 319... 

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