High performance capillary electrophoresis columns

Nelson, R. J., Paulus, A., Cohen, A. S., Guttman, A., and Karger, B. L., Use of Peltier thermoelectric devices to control column temperature in high-performance capillary electrophoresis, /. Chromatogr., 480, 111, 1989. 

Thormartn, W., Meier, R, Marcolli, C., and Binder, F., Analysis of barbiturates in human serum and urine by high-performance capillary electrophoresis-micellar electrokinetic capillary chromatography with on-column multiwavelength detection, /. Chromatogr., 545, 445, 1991. 

Organolead and organoselenium compounds were separated satisfactorily by high-performance capillary electrophoresis, using /1-cyclodextrin-modified micellar electroki-netic chromatography with on-column UVV detector set at 210 nm130. 

CEC is often inappropriately presented as a hybrid method that combines the capillary column format and electroosmotic flow employed in high-performance capillary electrophoresis with the use of a solid stationary phase and a separation mechanism, based on specific interactions of solutes with the stationary phase, characteristic of HPLC. Therefore CEC is most commonly implemented by means typical of both HPLC (packed columns) and CE (use of electrophoretic instrumentation). To date, both columns and instrumentation developed specifically for CEC remain scarce. 

Tsuji, K. (1991). High performance capillary electrophoresis of proteins. Sodium dodecyl sulphate-polyacrylamide gel-filled capillary column for the determination of recombinant biotechnology-derived proteins. J. Chromatogr. 550, 823-830. 

Ulfelder KJ, Dobbs M, Liu M-S (1995) Identification of PCR products by pre-column and on-column hybridization using capillary electrophoresis. Poster P-224, presented at the Seventh International Symposium on High Performance Capillary Electrophoresis, WUrzburg, Germany. 

Rush, R.S. Cohen, A.S. Karger, B.L. Influence of column temperature on the electrophoretic behavior of myoglobin and a-lactalbumin in high-performance capillary electrophoresis. Anal. Chem. 1991, 63, 1346-1350. 

Huang, X., You, T., Li, T., Yang, X., and Wang, E., End-column electrochemical detection for aromatic amines with high performance capillary electrophoresis/end-column/electrochemical detection/aromatic amines/capillary electrophoresis. Electroanalysis, 11, 969-972, 1999. 

Zhang S.S.jYuanZ.B., Liu H.X., Zou H., Xiong H., WuYJ., Analysis of acyclovir by high performance capillary electrophoresis with on-column amperometric detection. Electrophoresis, 21, 2995-2998... 

SFC uses the same stationary phases as FIPLC. Selectivity is similar but not identical. One of the greatest differences from n-FIPLC is in speed. SFC optimum flow is inherently three to five times faster, while peak shapes are often significantly better. Unlike n-HPLC, SFC reequilibrates after passage of only a few column volumes. Overall, SFC is often much more than 10 times faster than FIPLC. A fast chiral SFC separation is shown in Figure 4. Note that the column is not high speed but a standard 4.6x250 mm column with 10 pm particles. Some industrial pharmaceutical companies have dropped HPLC for chiral analysis and use SFC for less polar solutes, and high-performance capillary electrophoresis (HPCE) for water-soluble solutes. Unlike HPCE, SEC is scalable. 

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