High-magnification objective

With ultra-high magnification, objects appear more similar. All objects... 

As a next step, Carr et al. proposed using a 74x high-magnification objective such that each pixel of the FPA detector sampled a region significantly smaller than the diffraction limit, as would be needed to successfully achieve spatial... 

This makes it possible to reach a high magnification of details. The magnification can amount up to 100 1. The factor of magnification V of an object amounts, with the values shown in figure 2, according to equation 2 to ... 

Equations 1 and 2 shows that the distance b between the radiation source and the object has big influence on the X-ray image [8]. Therefore you have to find a compromise between a small geometrical unsharpness and a sufficient high magnification to archive an optimal... 

Scanning electron microscopy (SEM) can produce images of surface and objects at high magnification. If the scanning of the electron beam is also coupled to detection of x-rays from the electron impact on the... 

Fig. 3. Head of a Hydra oligactis labeled with monoclonal antibody DBS. (A) Neurons of hypostome and perihypostomal ring (left) and base of tentacle (right) at high magnification (x 20 objective) note considerable out-of-focus labeling of the tentacle (B) same specimen as in (A) but at lower magnification (x 10 objective) although the hypostome is out of focus, the neuronal net of the tentacles is much clearer. Scale bars = 50 pm.

The preparation is then passed under the objective, being kept in focus by means of slight, continuous movements of the micrometer screw this movement is very useful, especially with high magnifications, as it permits the images in different planes to be observed. 

PET film (35 pm) was necessary in order to allow the use of a high-magnification microscopic objective needed for optical trapping. This cell selection usually took about 5 min. Subsequent cell lysis and CE separation of the cell contents were... 

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