High Effectiveness of an HCA Cell Model in Predictive Toxicology

High Effectiveness of An HCA Cell Model in Predictive Toxicology 

HCA refers to the application of a recently developed technology that consolidates and automates microscopy, cytochemistry, imaging and bioinfomatics. These are applied to cells in various microtiter plate formats or on glass slides. Multiple morphologic features and fluorescence signals can be measured simultaneously. 

The measurements can be made at various levels from that of subcellular organelles, to single cells, cell populations and even tissues. They can be made on either fixed cells or on live cells that are incubated under physiological conditions. They can be made in end point assays or kinetically, in real time. Compared to previous manual methods, automation provides a marked improvement in the capacity for sample and experiment throughput, the precision of measurement and in the sheer number and diversity of parameters measureable for an experiment. Consolidation of the technical capabilities allo vs unparalleled vhthin-experiment, cross-comparisons of biochemical, morphological and functional parameters. Compared to flo v cytometry it offers substantially greater analytic capability for morphometric and kinetic parameters, although for substantially lo ver numbers of cells. 

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