Hexokinase molecular weight

Hexokinase has a molecular weight of 102,000 and is composed of two identical subunits of 51,000 molecular weight each (20-22). In yeast, hexokinase exists as a mixture of two isoenzymes (23). These forms have been named A and B (23). These isoenzymes can be separated by chromatography and have been found to be chemically different (23, 24). Some of the earlier work with hexokinase was done with preparations that contained a mixture of isoenzymes. Work also was done with enzyme that was proteolytically modified. These variations undoubtedly have been responsible for some of the controversy concerning the properties of this enzyme. 

Schulze et al. [135] developed fused-silica chips dynamically coated with hydroxypropylmethyl cellulose and utilized them for the separation of aromatic low molecular weight compounds such as serotonin, propranolol, a diol, and tryptophan. The authors used deep UV laser-induced fluorescence detection for these compounds. Schuchert-Shi et al. [136] identified ethanol, glucose, ethyl acetate, and ethyl butyrate, byproducts obtained in enzymatic conversions using hexokinase, glucose oxidase, alcohol dehydrogenase, and esterase. The authors reported that the quantification for ethyl acetate was possible using contactless conductivity detection. Hu et al. [137] described the separation of reaction products of (3-thalassemia in a multiplex primer-extension reaction using NCE. The method developed was used for patient samples and the results coincided with those of a detection kit. 

---