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Heterobifunctional reagents reactive

The greatest degree of control in crosslinking procedures is afforded using heterobifunctional reagents (Chapter 5). Since a heterobifunctional crosslinker has different reactive groups on either end of the molecule, each side can be directed specifically toward different functional... [Pg.33]

HSAB (N-hydroxysuccinimidyl-4-azidobenzoate) is a heterobifunctional reagent containing an amine-reactive NHS ester on one end and a photoreactive phenyl azide group on the other end... [Pg.308]

Sulfo-SAMCA, sulfosuccinimidyl-7-azido-4-methylcoumarin-3-acetate, is a heterobifunctional reagent similar in design to SAED (Section 3.9, this chapter) (Thermo Fisher). One end of the crosslinker contains an amine-reactive sulfo-NHS ester, while the other end is an AMCA derivative (Chapter 9, Section 3) that contains a photosensitive phenyl azide group. Unlike... [Pg.319]

The most common type of heterobifunctional reagent used for the activation of lipid components includes the amine- and sulfhydryl-reactive crosslinkers containing an N-hydroxysuccinim-ide (NHS) ester group on one end and either a maleimide, iodoacetyl, or pyridyl disulfide group on the other end (Chapter 5, Section 1). Principle reagents used to effect this activation process include SMCC (Chapter 5, Section 1.3), MBS (Chapter 5, Section 1.4), SMPB (Chapter 5, Section 1.6), SIAB (Chapter 5, Section 1.5), and SPDP (Chapter 5, Section 1.1). Other... [Pg.871]

The following heterobifunctional reagents are organized according to their reactivities. The majority are commercially available and their properties well documented in the literature. [Pg.249]

Perhaps the most popular heterobifunctional reagents are those that contain amine-reactive and sulfhydryl-reactive ends. The amine-reactive group is usually an active ester, most often an NHS ester, while the sulfhydryl-reactive portion may be one of several different functional groups. The amine-reactive end of these cross-linkers is... [Pg.249]

Sulfosuccinimidyl-2-(w-azido-o-nitrobenzamido)-ethyl-l,3 -dithiopropionate (SAND) is a heterobifunctional reagent containing an amine-reactive sulfo-NHS ester at one end and a photoreactive phenylazide group on the other (Pierce). The presence of the sulfonate group on the NHS ring lends water solubility to the reagent due to its... [Pg.283]

Sulfosuccinimidyl 4-(p-azidophenyl)butyrate (sulfo-SAPB) is a photoreactive heterobifunctional reagent containing an amine-reactive sulfo-NHS ester at one end (Pierce). [Pg.286]

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See also in sourсe #XX -- [ Pg.297 ]



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Heterobifunctional reagents

Heterobifunctional reagents sulfhydryl-reactive

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Reagent reactivity

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