Crosslinkers sulfhydryl reactive

Although acryloyl crosslinking agents have not been common, the reactive group has found use in the design of the sulfhydryl-reactive fluorescent probe, 6-acryloyl-2-dimethylaminonaph-thalene (acrylodan Molecular Probes) (Epps et al., 1992 Yem et al., 1992). 

The differences within these families of reagents generally relate to the length of the spacer or bridging portion of the molecule. Occasionally, the bridging portion itself is designed to be cleavable by one of a number of methods (Chapter 8). The great majority of homobifunctional, sulfhydryl-reactive crosslinkers mentioned in the literature are not readily available from commercial sources and would have to be synthesized to make use of them. The ones listed in this section are obtainable from Thermo Fisher. 

Figure 4.17 DPDPB is a sulfhydryl-reactive crosslinker that forms disulfide bonds with thiol-containing molecules. The conjugates may be disrupted using a disulfide reducing agent such as DTT.

Figure 4.35 Benzyl halides and halomustards can be used as crosslinking agents reactive toward sulfhydryl groups.

---