Herbicides Transgenic rice plants

Kawahigashi, H., Hirose, S., Inui, H., Ohkawa, H., and Ohkawa, Y., 2005, Enhanced herbicide cross-tolerance in transgenic rice plants co-expressing human CYPlAl, CYP2B6, and CYP2C19, Plant Sci. 168 773-781. 

Li, Z.J., Hayashimoto, A., and Murai, N. 1992. A sulfonylurea herbicide resistance gene from arabidopsis-thaliana as a new selectable marker for production of fertile transgenic rice plants. Plant Physiol., 100, 662-668. 

Kawahigashi H, Hirose S, Ohkawa H, Ohkawa Y (2003) Transgenic rice plants expressing human CYPlAl exude herbicide metabolites from their roots. Plant Sci 165 373-381... 

Evaluation of Herbicide Metabolism in Transgenic Rice Plants Expressing CYPlAl and CYP2B6... 

We have produced transgenic rice plants eiqnressing CYPIAI and CYP2B6 these transgenic rice plants showed remarkable cross-tolerance toward herbicides and enhanced herbicide detoxification, a property that should be useful in phytoremediation. 

Figure 2, Phytotoxicity of various herbicides toward CYPlAl and CYP2B6 transgenic rice plants in germination tests in MS medium, C, Nipponbare without herbicide (control) N, Nipponbare with herbicide lA, CYPlAl plants with herbicide 2B, CYP2B6 plants with herbicide.

These results indicate that exogienous CYP2B6 proteins enhanced the metabolism of herbicides in transgenic rice plants and had a high potential to metabolize chemical conqmunds in vivo in the xenobiotic pathway. 

Transgenic rice plants expressing CYP2B6 rapidly decreased the amount of metolachlor present in both the plants themselves and the culture medium. Thus, such transgenic plants should be inq>ortant not only for developing herbicide-tolerant cultivars, but also for decreasing herbicide residues in rice. 

Table I. Tolerance of Transgenic CYPIAI and CYP2B6 Rice Plants to Various Herbicides in Germination Tests...

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