Hepatocyte paraffin

Hepatocyte paraffin 1 (Hep Pari) is the most sensitive and highly specific marker of hepatocytic differentiation in paraffin embedded tissue. The antibody is directed against a mitochondrial antigen present within hepatocytes and shows a characteristic granular cytoplasmic staining (Fig. 8.18). The antibody HepPar-1 is 79% specific for hepatic differentiation, and sensitivity is However,... 

Wermerberg AE, Nalesnik MA, Coleman WB. Hepatocyte paraffin 1 a monoclonal antibody that reacts with hepatocytes and can be used for differential diagnosis of hepatic mmors. Am J Pathol. 1993 143 1050-1054. 

Pitman MB, Triratanachat S, Young RH, Oliva E. Hepatocyte paraffin 1 antibody does not distinguish primary ovarian tumors with hepatoid differentiation from metastatic hepatocellular carcinoma. Int J Gynecol Pathol. 2004 23 58-64. 

Lugli A, Tornillo L, Mirlacher M, et al. Hepatocyte paraffin-1 expression in human normal and neoplastic tissues Tissue microarray analysis on 3,940 tissue samples. Am J Clin Pathol. 2004 122 721-727. 

Maitra A, Murakata LA, Albores-Saavedra J. Immunoreactivity for hepatocyte paraffin 1 antibody in hepatoid adenocarcinomas of the gastrointestinal tract. Am J Clin Pathol. 2001 115 689-694. 

Interestingly, there is consistent immunola-beling with hepatocyte paraffin-1 (Hep Par-1) antibodies however, in situ hybridization for albumin, a more specific test for hepatocellular differentiation, is consistently negative." ... 

Zatloukal K, Stumptner C, Fuchsbichler A, et al. The keratin cy-toskeleton in liver diseases. The diagnostic value of hepatocyte paraffin antibody 1 in differentiating hepatocellular neoplasms from nonhepatic trrmors a review. J Pathol. 2004 204 367-376. 

Fasano M, Theise ND, Nalesnik M, et al. Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen. Mod Pathol. 1998 11 934-938. 

Livers of one experimental group were extracted and fixed in formalin. After fixing, liver pieces were embedded in paraffin and then slices of up to 10 pm thickness were made. The slices were pigmented by hematoxylin-eosin. An average area of a single hepatocyte as well as cell quantity in various mitosis phases were calculated in terms of a thousand elements under microscopical investigation. 

Fig. 12. Paraffin sections of rat liver, periodic acid Schiff stain X22. A, normal liver of control animal killed 24 h after injection of 1 ml of seasame oil i.p. B, extensive centrolobular necrosis of parenchymal cells in rat killed 24 h after administration of high dose of bromobenzene (0.2 ml i.p.). C, Liver 24 h after lower dose of bromobenzene (0.03 ml i.p.). The centrolobular areas exhibit some small patches of round cell infiltration and decreased glycogen staining in the cytoplasm after hepatocytes but little necrosis. D, Extensive centrolobular necrosis after administration of the same low dose of bromobenzene (0.03 ml i.p.) to a rat treated with pheno-barbital (20 mg/kg) for 3 days in order to induce hepatic microsomal enzymes. After Brodie et al...

---