Heparin Sulphatases

Heparin Sulphatases.—Mucosal scrapings from rats, stomachs contain heparin sulphatase activity. ... 

In the field of aldobiouronic acid derivatives an ingenious method has been devised for the preparation of radioactive disaccharides for use as substrates for o-L-iduronidase, jS-D-glucuronidase, and 2-sulpho-L-iduronate 2-sulphatase. Heparin was cleaved by the deamination procedure and the products were reduced using sodium borotritide to afford access to a-L-idopyranosyluronic and j3-D-glucopyranosyluronic acid derivatives of 2,5-anhydro-D-[l- H]mannitol. Acidic treatment in methanol of the water-soluble polysaccharides of wobaku wood, followed by acetylation, have led to the isolation of the anhydride derivative (53) which was concluded to arise by way of the corresponding aldo-... 

Chapter 9. A substrate used in the assay of L-iduronic acid 2-sulphate sulphatase was obtained from sodium heparinate by methanolysis followed by nitrosation. Disaccharide monosulphates presumed to contain D-(methyl a-L-idopyranosiduronate-2-sulphate)-(l 4)-2,5-anhydro-D-mannose were isolated by gel filtration. Brucine L-guluronate and o-mannuronate, on the other hand, were obtained by treatment with brucine of the barium salts of the acids formed on hydrolysis of alginic add. ... 

Sodium heparinate has been treated sequentially with methanolic hydrogen chloride and nitrous acid to produce 0-(methyl a-L-idopyranosyluronate 2-sulphate-(l -> 4)-2,5-anhydro-D-mannose. This disaccharide can be used for direct measurement of L-iduronic acid 2-sulphate sulphatase, an enzyme which is defective in Hunter s syndrome (mucopolysaccharidosis II). 

L-Iduronic acid 2-suIphate Sulphatase.—Commercially available sodium heparinate has been sequentially treated with methanolic 0.06 molar hydrogen chloride and nitrous acid. The non-degraded material was separated by gel filtration from the nonsulphated and monosulphated disaccharides produced. The latter ones, obtained in 10% yield, have been used as a substrate for the... 

Glycopeptide-linkage hydrolases a-L-Guluronan lyase Heparin hydrolase Hyaluronate lyase Hyaluronidase L-Iduronic acid 2-sulphate sulphatase a-L-Iduronidase Inulinase Isoamylase Isopullulanase a-Lactalbumin Lactose synthase... 

Deaminative cleavage of heparin and reduction of the resulting disulphated disaccharide with sodium borotritide afforded 2,5-anhydro-4-0-(a-L-idopyran-uronosyl 2-sulphate)-D-[l- H]mannitol 6-sulphate, which was used as the substrate in an assay for a-L-idopyranosyluronic acid 2-sulphate sulphatase. Heparin has been labelled in vivo with S-sulphate. ... 

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