Heparin gels

The sorbed heparin fully retains its anticoagulant activity the thrombin time for both sorbed heparin and heparin in solution is 20 + 2 s/mg heparin. The clotting time of blood in contact with the gel is proportional to the amount of heparin sorbed and significantly higher than that characteristic for the non-heparinized gel. Heparin may be repeatedly sorbed after it has been removed with saturated NaCl solution. 

EN38 Haven, M.C., Earl, R.A., Baird, J.L. and Fellows, P.A. (1991). Examination of the reference range for asparate aminotransferase determined on the Ek-tachem 700 SR after collection in heparinized gel separator tubes. Clin. Chem. 37, 913-914, Abstr. 23. 

Heparin was released from 1% heparin-PVA gel "beads at 1.7 x 10 yg/g wet gel/min. This rate was determined from the approximately linear portion of the heparin content versus PBS (phosphate buffered saline, pH 7.4) wash time curve (between 300 and 500 hours) by using the toluidine blue method ( ). This release rate was 1/1000 of the minimum rate considered necessary for thromboresistance ( ). After washing, the remaining heparin content was determined indirectly by mass balance to be approximately 7.1 mg/g wet gel for 1% heparin gels and 14 mg/g wet gel for 2% heparin gels. 

J, which indicated a large amount of active thrombin was bound to the heparinized gel ( ). After passing antithrombin III through each column, colour yields of 18-485 were obtained, indicating significant inactivation of thrombin by antithrombin III. However, using a second set of heparin - PVA columns with antithrombin III loaded first onto the heparinized gel followed by thrombin and... 

However, it is clear that thrombin adsorbed by PVA-heparin is biologically active and is inactivated by antithrombin III presumably through the formation of a surface-bound heparin-thrombin-antithrombin III complex. Furthermore, the biological activity of the heparinized gel and the mechanism of thrombin inactivation by antithrombin III on heparin-PVA have been verified using clotting assays (3). 

Extraction of LDL and IDL by Heparin Gel Affinity Separation for Oxidative Susceptibility/Resistance Testing... 

Affi-Gel Heparin gel Cross-linked agarose Heparin 0 6 mg/mL... 

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