Hemolysis 748 INDEX

One index of the possible requirement for tocopherol by man may be obtained by determining what amounts are needed by a depleted subject to raise the plasma tocopherol and lower the peroxide hemolysis. This problem is made difficult by the fact that any judgment of the peroxide hemolysis or level of plasma tocopherol which is made immediately after the ingestion of a-tocopherol is partially invalidated by the ability of small amounts of tocopherol to remain in the blood stream for several days after ingestion. It takes more than 4 days for a single dose of tocopherol to reach a plateau... 

Refractometry Refractometry is a quick and reasonably accurate alternative to chemical analysis for serum total protein when a rapid estimate is required. The refractive index of water at 20°C is 1.330 if solute is added to the water, the refractive index of a dilute solution increases linearly and proportionally to the solute concentration at higher concentrations of dissolved solids (50-200gl ), the increase is nearly linear. Temperature affects appreciably the refractive index of a solution, so refracto-meters for clinical use compensate for temperature effects. Serum contains dissolved solids in concentrations of 80-100 gl, most of which are proteins. In the refractometry of serum, it is assumed that the concentration of inorganic electrolytes and nonprotein organic compounds does not vary appreciably from serum to serum and that the differences in the refractive index reflect primarily the differences in protein concentrations. The assumption has been shown to be reliable for clear, nonpigmented samples, but hemolysis, lipemia, icterus, and azotemia produce erroneously high results. The method cannot be used for urine protein measurement because of excess solutes in relation to the protein. 

Erythrocyte hemolysis. The experiments were performed in PBS using a static method according to [12]. The degree of erythrocyte lysis due to sample activity was calculated as the hemolytic index %H = where Hb is the... 

Erythrocyte hemolysis. Hemolysis experiments were performed in PBS using static method, as previously described [2], The degree of hemolysis due to sample activity was calculated as the hemolytic index %H = (Hb - Hbo/Hb,ot) 100%, where Hb is the total amount of released hemoglobin in the assayed sample, Hbo the amount released due to basal hemolysis (200pl erythrocytes incubated with 50pl PBS), and Hb,ot the total amount of released hemoglobin in fully disrupted erythrocytes (0.2 ml erythrocytes in 19.8 ml H2O MilU Q). 

Minimal hemolysis and formed element damage (Index of hemolysis <0.030)... 

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