Hemolysis commercial

The. animal work will be described only sufficiently to allow the reader to appreciate lietter the discussion of the human studies. When it became apparent in 19.55 that the peroxide hemolysis test was dependent not only on the tocopherol level of the blood but also upon the level of linoleic acid (and other autoxidizable components) in the stroma of the erythrocyte, animal experiments were designed to obtain more exact correlations between tocopherol needs and linoleic acid intake. This relationship between linoleic acid content of the diet and the incidence of chick encephalomalacia (Century and Horwitt, 19.58) was not recorded until later (Century et al., 19.59 Century and Horwitt, 19.59) when observation of cerebellar encephalomalacia in an infant that had been fed a commercial cottonseed oil preparation intravenously came to our attention (Horwitt and Bailey, 1959). In the meantime, there had been a number of reports to certify the relationship between linoleic acid consumption and chick encephalomalacia (Dam et al, 19.58 Machlin and Gordon, 1960). With the advent of better gas chromatographic techniques, it was soon possible to show that the linoleic acid content of the cerebellum was diet dependent (Horwitt et al., 1959 Witting et al., 1961). The marked effects of diet on the fatty acids of the mitochondria of chick brains has also been reported (Horwitt, 1981a). The levels of linoleic acid are much lower in brain tissues than in any tissue analyzed to date and this relatively low linoleic acid level may be considered a characteristic of brain tissue. The significance of this difference is not known. It is of interest to note that the current interpretations of the effect of more unsaturated fats on the production of chick encephalomalacia were anticipated by Dam in 1944. 

Subjects whose tocopherol levels were 0.28, 0.18 and 0.21 mg% and whose peroxide hemolysis levels were 71, 87, and 84%, respectively, were taken off the stripped oxidized corn oil and placed on commercial corn oil for 3 months. The slightly improved results obtained were probably largely a function of the uncleared tocopherol from the commercial corn oil remaining in the plasma at the time the sample was taken, so for the next 20 days, stripped corn oil which had not been oxidized was substituted for the commercial corn oil, during which time a significant rise in the peroxide hemolysis results were obtained. 

Effect of Substituting either Commercial Corn Oil (Mazola) or Stripped Corn Oil for Oxidized Stripped Corn Oil on Plasma Tocoperhol AND Peroxide Hemolysis of Depleted Subjects... 

Blood and hair samples are often analyzed in order to estimate exposure of humans to mercvny and its compounds. Blood should be taken by venipuncture. Since some commercial containers may contain mercury compounds added as preservatives it is advisable to check each commercial batch before use. The samples should be refrigerated but not frozen, as it is sometimes useful to measure mercvny in plasma and red blood cells separately. The separation of plasma and red blood cells should be performed as soon as possible to avoid hemolysis of the sample. If extensive hemolysis has occurred, the sample should be homogenized before an aliquot is taken for analysis. Blood samples may also be heparinized for total blood, serum, and red blood cell analyses. If unavoidable, samples may be stored deep frozen. However, repeatedly frozen and unfrozen blood samples showed a remarkable decrease in methylmercury concentrations. There is some evidence that methyl-mercvny may be destroyed during lyophilization of blood samples. 

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