Electrophoresis hemoglobin

Table 65-1 shows the clinical features of SCT and SCD. SCD usually is identified by routine neonatal screening programs using hemoglobin electrophoresis, thin-layer isoelectric... 

Hemoglobin electrophoresis takes advantage of the differences in pi values between HbA and HbS (GluSVal glutamate at position 6 has been replaced by valine). 

Two modifications of classical hemoglobin electrophoresis are also useful for studying these proteins isoelectric focusing and electrophoresis of individual globin chains, with or without the heme. These techniques can be used to identify some hemoglobins that are not separable by electrophoresis of the complete hemoglobin tetramers. 

An adolescent presents with shortness of breath during exercise and is found to be anemic. A hemoglobin electrophoresis is performed that is depicted in the figure below. The adolescents sample is run with controls including normal, sickle trait, and sickle cell anemia, and serum. The adolescent is determined to have an unknown hemoglobinopathy. Which one of the lanes contains the adolescent s sample ... 

Glucose-6-phosphate dehydrogenase Ham s test Haptoglobin Hematocrit Hemoglobin electrophoresis... 

Flemoglobin (Hb) carries the vital oxygen to the different tissues. Its concentration is used clinically as an indicator of the different types of anemia. Hemoglobin is a tetramer composed of 2a chains and 2 3 chains. The P chain is more susceptible than the a chain for amino acid substitution (mutations), which results in different variants often present in special populations. Most of the variants are harmless and of research interest. However, few such as Hb S are associated with severe anemia, decreased capacity to carry oxygen, and altered red blood cell shape. The most encountered variants of hemoglobin are A, E, S, C, and E. Hemoglobin electrophoresis is carried out for two purposes detection of Hb variants and detect the presence of thalassemias (decreased synthesis of one of the Hb chains). 

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