HemA gene

Hungerer C, Weiss DS, Thauer RK, Jahn D. The hemA gene encoding glutamyl-tRNA reductase from the archaeon Methanobacterium thermoautotrophicum strain Marburg. Bioorg. Med. Chem. 1996 4 1089-1095. 

Stavudine (d4T) is a nucleoside reverse transcriptase inhibitor. While it has only minor hema-totoxic potential, the dmg is markedly neurotoxic, causing dose-limiting peripheral neuropathy. Resistance occurs via mutations in the pol gene, which encodes for several proteins including reverse transcriptase. The answer is (B). 

Amphiphilic block copolymers with covalentiy linked hydrophilic and hydrophobic blocks capable of forming micelles can be conveniently prepared by ATRP techniques [86-88]. A representative example is the synthesis of polymethacrylate based pentablock copolymers (PBPs) via consecutive ATRP technique reported by Xu et al. [89] for non-viral gene delivery applications. In their approach, PBPs of poly(HEMA)-Z7-poly(DMAEMA)-fc-PEG-fc-poly(DMAEMA)-fo-poly(HEMA) were prepared via consecutive ATRPs from di-2-bromoisobutyryl-terminated PEG (Br-PEG-Br) (Fig. 2.24). The very low in vitro cytotoxicity and excellent gene transfer efficacies exhibited by these PBPs demonstrated the versatility of the ATRP technique to tailor the polymer structural-property characteristics for such applications. 

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