Helios Gene Gun

Martiniuk, F., Chen, A., Mack, A., Donnabella, V., Slonim, A., Bulone, L., Arvanitopoulos, E., Raben, N., Plotz, P. and Rom, W. N. (2002). Helios gene gun particle delivery for therapy of acid maltase deficiency. DNA Cell Biol. 21, 717-725. 

Belyantseva IA (2009) Helios gene gun-mediated transfection of the inner ear sensory epithelium. Methods Mol Biol 493 103-123... 

Finally close the nitrogen cylinder and cut the Tefzel tube at the right length to enter in the barrel of the Helios Gene Gun . 

Distance between the Helios Gene Gun barrel and target 16 mm. 

Insert cartridges in the barrel of the Helios Gene Gun . Connect the Helios Gene Gun with the helium cylinder, and select an operating pressure of 160 psi. Place the barrel liner over the target (i.e., the dish containing the coverslip with attached cultured neurons or the petri dish containing the slice on the Millicell-CM insert) at a distance of 1.6 mm with a spacer. 

Give one single shot to each culture dish. Culture dishes must be shot under a laminar flow hood. To avoid contamination, the Helios Gene Gun barrel must be sterilized with absolute ethanol immediately before use and culture dish lids opened only for the time strictly necessary for shooting. 

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