Harris, Elisa

Harris, Elisa D., CBW Arms Control A Regime Under Attack , Arms Control Today, September 1986. 

The assessment of DNA adducts may provide a sensitive indicatCH of previous exposure. The enzyme-linked immunosorbent assay (ELISA) has a lower limit of detection of about 0.08 femtomol per microgram of DNA (Perera et oL, 1982). This assay requires (1) the development of an antibody specific for a certain chemical metabolite bound covalently to DNA and (2) the isolation of DNA from some tissue sample, ag., skin biopsy, or lymphocytes of an exposed individual. It is anticipated that further refinement of such immunologic techniques may lower the threshold of sensitivity by one or two orders of magnitude. One such refined test is the ifitrasensitive ymatic radioimmunoassay (USE-RIA), purported to be about five times more sensitive than ELISA (Hsu et oL, 1981 Shamsuddin et oL, 1985 Harris et oL, 1985). Quantification by the development of monoclonal antibodies to aflatoxin Bj metabolites bound to DNA (Groopman et oL, 1982 Sizaret et oL, 1982) has now been reported. 

R3. Reckel, R. P., Harris, J., Botsko, E., Wellerson, R., and Varga, S., The detection of circulating immune complexes containing Clq and IgG using a new rapid ELISA test system. Diagn. Immunol, in press (1984). 

The ELISA technique is used for detection of antibodies in serum bound to BPDE-DNA adducts. The USERIA method involves measuring the immunological response of BPDE-DNA in the presence of rabbit anti-serum. Several researchers have employed the immunoassay techniques for detecting PAH-DNA adducts at 10 mol levels in the blood and tissues of humans occupationally exposed to PAHs (Harris et al. 1985 Haugen et al. 1986 Newman et al. 1988 Perera et al. 1988 Shamsuddin et al. 1985 Weston et al. 1988). 

Coal tar creosote Separation of lymphocyte cells and isolation of BPDE-DNA adduct by standard treatments. ELISA or USERIA and SLS 0.006-0.23x 10 15 mol BPDE per pg DNA No data Harris et al. 1985... 

Allen, Greirson, and Harris, from the Agricultural Chemistry Laboratory of the Chemistry Centre in Western Australia, use the following steps for lupine alkaloid extraction in ELISA detection ... 

Allen DG, Greirson BN, Harris DJ. An enzyme immunosorbent assay (ELISA) for lupin alkaloids Comparison with gas chromatography. In von Baer D, editOT. International lupin Association sixth international lupin conference proceedings. November 25—30, 1990. Temuco-Pucon, Chile Asociacion Chilma del Lupino 1991. p. 406. 

Elisa D. Harris, Sverdlovsk and Yellow Rain Two cases of Soviet Noncompliance , International Security, Spring 1987, p. 75. 

UN Press Release DC/892 of 21 March 1980. The official explanation for the outbreak was subsequently elaborated in a Soviet journal in an article dated August 1979 (that is, prior to the US allegations). Whether this was the actual date of the article s submission is not known. See Elisa Harris, op. cit., p. 50n36. 

New York Times, 25 November 1981. Donald E. Ellis, Report on Scientific Exchange Visit... , cited Elisa Harris, op. cit., pp. 53-4. 

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