Hansenula polymorpha growth

Yeasts Easy to grow in large-scale volumes Able to glycosylate. Improved glycosylating yeast Pichiapastorisand Hansenula polymorpha Easy to achieve secretion of recombinant proteins into growth medium Gene expression can be difficult to control Some post-translational modifications may not be correct... 

With increasing amounts of AFA in Hansenula polymorpha cultivation medium, kLa passes a maximum at 1.0 %o (w/w) Desmophen. However, gas holdup attains the maximum at 10%o (w/w) Desmophen [41]. The surface tension diminishes and the surface viscosity increases with increasing antifoam concentration in distilled water and in BSA solution. Foaminess 2 drops from 200 to 1 s already at low concentrations of Desmophen (< 1 mgl" ). In a cultivation media of Hansenula polymorpha and E. coU, the surface tension and surface viscosity remain constant during the entire cultivation time in the presence of Desmophen [22]. As already pointed out, the maximum of foaminess which arises at the transition from the growth phase to the production phase during the production of penicillin V by Penicillium chrysogenum can be eUminated by adding Desmophen to the cultivation medium. The foaminess is reduced from 400 to 100 s [26]. 

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